Cloning, characterization, and expression of the nitric oxide-generating nitrite reductase and of the blue copper protein genes of Achromobacter cycloclastes

Jang Yi Chen, Wei Chao Chang, Tschining Chang, Wen Chang Chang, Ming Yih Liu, William J. Payne, Jean LeGall

研究成果: 雜誌貢獻文章同行評審

23 引文 斯高帕斯(Scopus)

摘要

The nitrite reductase (NIR) and blue copper protein (BCP) genes have been cloned from Achromobacter cycloclastes and characterized. NIR gene encodes a protein of 378 amino acid residues including a putative signal peptide of 37 residues. BCP gene encodes a protein of 148 residues with a 24-residue signal peptide. The DNA-derived amino acid sequence of NIR is in complete agreement with that from Edman degradation and the DNA coding sequence of BCP is also consistent with its partial N-terminal amino acid sequence. Both genes contain their own FNR box in the 5' upstream region and a TA-rich region that could be the transcription start site. These two genes are separated by at least 10 kb. Based on these observations it is very likely that these two genes, although functionally related, are regulated independently. Both proteins could be expressed in E. coli, and both of the expressed proteins could be recognized by their respective antisera. The expressed NIR demonstrates full enzymatic activity. The similarity of both proteins to the counterparts from Alcaligenes faecalis S-6 is discussed.
原文英語
頁(從 - 到)423-428
頁數6
期刊Biochemical and Biophysical Research Communications
219
發行號2
DOIs
出版狀態已發佈 - 2月 15 1996
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學

指紋

深入研究「Cloning, characterization, and expression of the nitric oxide-generating nitrite reductase and of the blue copper protein genes of Achromobacter cycloclastes」主題。共同形成了獨特的指紋。

引用此