Chronic stress affects tyrosine phosphorylated protein expression and secretion of male rat epididymis

Supatcharee Arun, Arada Chaiyamoon, Natthapol Lapyuneyong, Sudtida Bunsueb, Alexander Tsang Hsien Wu, Sitthichai Iamsaard

研究成果: 雜誌貢獻文章同行評審

7 引文 斯高帕斯(Scopus)


Chronic stress (CS) is shown to decrease the semen quality with changed expression of tyrosine phosphorylated (TyrPho) proteins in testicular and seminal tissues. However, the alterations of such proteins and fluid contents in the epididymis, producing sperm maturation factors, have never been reported. Sixteen adult rats were randomly divided into 2 groups (n = 8). The control animals were not subjected to stressors whereas CS rats were immobilised within restraint cage (4 hr/day) before cold forced-water swimming (15 min/day) for 60 days. Corticosterone, testosterone, blood glucose level (BGL), malondialdehyde (MDA) and biochemical components in epididymal fluid were assayed. Expressions of heat shock protein 70 (HSP-70), androgen receptor (AR) and TyrPho protein were investigated in epididymal tissue and fluid. Significantly, CS increased the corticosterone and BGL but decreased testosterone and epididymal substance levels. MDA level in tail epididymal fluid and HSP-70 expression in both regions of epididymal tissues and fluids, except in head epididymal fluid of CS were increased. Epididymal tissues showed the decrease of AR expression. Presence and changes of many TyrPho proteins were observed in CS. In conclusion, CS could affect functional proteins particularly TyrPho in epididymis, resulted in low semen quality.
出版狀態已發佈 - 4月 2021

ASJC Scopus subject areas

  • 內分泌
  • 泌尿科學


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