TY - JOUR
T1 - Chitosan-based polyelectrolyte complex scaffolds with antibacterial properties for treating dental bone defects
AU - Wu, Hong Da
AU - Ji, Dian Yu
AU - Chang, Wei Jen
AU - Yang, Jen Chang
AU - Lee, Sheng Yang
PY - 2012/2/1
Y1 - 2012/2/1
N2 - The aim of this study was to develop an antibacterial polyelectrolyte complex (PEC) scaffold for treating dental bone defects. The PEC scaffold was composed of chitosan (CS), γ-polyglutamic acid (γ-PGA), and carboxy-methyl-cellulose (CMC). The resulted network structures formed via electrostatic crosslinking were characterized by using FTIR, gel content, equilibrium swelling ratio, volume change, and SEM test. The antibacterial property, cell cytotoxicity, and in vivo biocompatibility tests were conducted according to an agar diffusion method, ISO10993-5, and ISO 10993-6, respectively. The resulted specimens showed an interconnected pore structure with pore sizes ranging 100-500 μm. The equilibrium swelling ratio, volume change, and antibacterial property were inversely proportional to the gel content. The PEC-2 scaffold composed of 8 wt.% CS, and 2 wt.% γ-PGA + 2 wt.% CMC had more-suitable gel properties (gel content of 55.3 ± 1.1 wt.% and volume change of 97.7 ± 1.4 v/v%) with inhibition zones of 14.4 ± 0.3 mm for Escherichia coli and 13.0 ± 0.7 mm for Staphylococcus aureus. The cytotoxicity and cell attachment tests of the PEC scaffolds showed satisfactory cell compatibility. Moreover, the in vivo biocompatibility test of the PEC scaffolds revealed little foreign body reaction. For this reason, the newly developed antibacterial PEC scaffold may be a good alternative for dental applications.
AB - The aim of this study was to develop an antibacterial polyelectrolyte complex (PEC) scaffold for treating dental bone defects. The PEC scaffold was composed of chitosan (CS), γ-polyglutamic acid (γ-PGA), and carboxy-methyl-cellulose (CMC). The resulted network structures formed via electrostatic crosslinking were characterized by using FTIR, gel content, equilibrium swelling ratio, volume change, and SEM test. The antibacterial property, cell cytotoxicity, and in vivo biocompatibility tests were conducted according to an agar diffusion method, ISO10993-5, and ISO 10993-6, respectively. The resulted specimens showed an interconnected pore structure with pore sizes ranging 100-500 μm. The equilibrium swelling ratio, volume change, and antibacterial property were inversely proportional to the gel content. The PEC-2 scaffold composed of 8 wt.% CS, and 2 wt.% γ-PGA + 2 wt.% CMC had more-suitable gel properties (gel content of 55.3 ± 1.1 wt.% and volume change of 97.7 ± 1.4 v/v%) with inhibition zones of 14.4 ± 0.3 mm for Escherichia coli and 13.0 ± 0.7 mm for Staphylococcus aureus. The cytotoxicity and cell attachment tests of the PEC scaffolds showed satisfactory cell compatibility. Moreover, the in vivo biocompatibility test of the PEC scaffolds revealed little foreign body reaction. For this reason, the newly developed antibacterial PEC scaffold may be a good alternative for dental applications.
KW - Antibacterial
KW - Biocompatible
KW - Bone regeneration
KW - Chitosan
KW - Polyelectrolyte complex
KW - γ-polyglutamic acid
UR - http://www.scopus.com/inward/record.url?scp=84855291291&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84855291291&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2011.10.020
DO - 10.1016/j.msec.2011.10.020
M3 - Article
AN - SCOPUS:84855291291
SN - 0928-4931
VL - 32
SP - 207
EP - 214
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
IS - 2
ER -