Chitinase activity of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57)

Wen Chi Hou, Ying Chou Chen, Yaw Huei Lin

研究成果: 雜誌貢獻文章同行評審

19 引文 斯高帕斯(Scopus)

摘要

Chitinase activity was detected in the crude extracts of different parts of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57) using commercial deacetyl glycol chitin as a substrate. The activity of chitinase in crude extracts, which was expressed as units/g fresh weight, was arranged in decending order of leaves > sprouts > peels of storage roots > peeled storage roots. Under field conditions, the chitinase activity of leaves was higher than vines. The chitinase activity of insect-bitten leaves was higher than that of the controls, and the chitinase activity of vines attached to insect-bitten leaves was also higher than that of vines attached to intact leaves. The molecular weight of main chitinase determined by activity staining was about 16 kDa. The optimum pH was 5.0 and the optimum temperature of chitinase activity was 25°C.

原文英語
頁(從 - 到)93-97
頁數5
期刊Botanical Bulletin of Academia Sinica
39
發行號2
出版狀態已發佈 - 1998
對外發佈

ASJC Scopus subject areas

  • 植物科學

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