TY - JOUR
T1 - Characterization of ovomucoid-specific T-cell lines and clones from egg-allergic subjects
AU - Eigenmann, Philippe A.
AU - Huang, Shau Ku
AU - Sampson, Hugh A.
PY - 1996/2
Y1 - 1996/2
N2 - In the pathogenesis of allergic reactions, T cells and cytokines play a major role. However, characterizations of food allergen-specific T cells are very limited. In this study, we screened the peripheral blood mononuclear cells (PBMC) of 14 patients for reactivity to ovomucoid (Gal d I), the major hen's egg allergen, and ovalbumin (Gal d II). Cell lines and clones specific to ovomucoid were generated from PBMC of four egg-allergic subjects, in order to study antigen domain specificity and cell cytokine production profiles. The results demonstrated, firstly, that egg-allergic patients respond to ovomucoid rather than to ovalbumin, and, secondly, that antigen specificity is predominantly directed toward the second and third domains of ovomucoid. The T-cell cytokine message was characterized by reverse transcriptase polymerase chain reaction (RT-PCR). Cell lines and clones from all four patients consistently expressed interleukin (IL)-5. IL-4, IL-13, and interferon-gamma were found to be expressed only by certain lines or clones. This observation suggests a central pathogenic role for IL-5 in food allergy-related symptoms.
AB - In the pathogenesis of allergic reactions, T cells and cytokines play a major role. However, characterizations of food allergen-specific T cells are very limited. In this study, we screened the peripheral blood mononuclear cells (PBMC) of 14 patients for reactivity to ovomucoid (Gal d I), the major hen's egg allergen, and ovalbumin (Gal d II). Cell lines and clones specific to ovomucoid were generated from PBMC of four egg-allergic subjects, in order to study antigen domain specificity and cell cytokine production profiles. The results demonstrated, firstly, that egg-allergic patients respond to ovomucoid rather than to ovalbumin, and, secondly, that antigen specificity is predominantly directed toward the second and third domains of ovomucoid. The T-cell cytokine message was characterized by reverse transcriptase polymerase chain reaction (RT-PCR). Cell lines and clones from all four patients consistently expressed interleukin (IL)-5. IL-4, IL-13, and interferon-gamma were found to be expressed only by certain lines or clones. This observation suggests a central pathogenic role for IL-5 in food allergy-related symptoms.
KW - Antigenic determinants
KW - Cytokines
KW - Food hypersensitivity
KW - Reverse transcriptase polymerase chain reaction
KW - T cells
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U2 - 10.1111/j.1399-3038.1996.tb00100.x
DO - 10.1111/j.1399-3038.1996.tb00100.x
M3 - Article
C2 - 8792379
AN - SCOPUS:0030011033
SN - 0905-6157
VL - 7
SP - 12
EP - 21
JO - Pediatric Allergy and Immunology
JF - Pediatric Allergy and Immunology
IS - 1
ER -