摘要
A lipoxygenase inhibitor in the cytosolic fraction of human epidermoid carcinoma A431 cells was characterized and purified. The cytosolic inhibitor lost the inhibitory activity upon heating at 75°C for 15 min or pretreating with 1 mg/ml trypsin at 37°C for 60 min. Cytosol, after dialysis, lost the inhibitory activity but its inhibitory activity recovered when 1 mM GSH was added to the dialysate. The inhibitory activity of cytosol was also abolished by treatment either with 1 mM iodoacetate at 4°C for 1 h or with 0.5 mM H2O2. The pI of the inhibitor was approx. 7.0. In addition to 12-lipoxygenase, the inhibitor inhibited the activities of 5-lipoxygenase and fatty acid cyclo-oxygenase in a cell-free system. The inhibitor was purified by a series of column chromatographies using CM Sephadex C-50, Sephadex G-100 SF and Mono P columns. A major 22 kDa protein was obtained that was distinct from selenium-dependent glutathione peroxidase.
原文 | 英語 |
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頁(從 - 到) | 193-198 |
頁數 | 6 |
期刊 | Biochemical Journal |
卷 | 327 |
發行號 | 1 |
DOIs | |
出版狀態 | 已發佈 - 1997 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 分子生物學
- 生物化學
- 細胞生物學