@article{f7f68bee3032419eb3d62b78ad85a8b0,
title = "Cell-based semiquantitative assay for sulfated glycosaminoglycans facilitating the identification of chondrogenesis",
abstract = "Glycosaminoglycans (GAGs), in particular chondroitin sulfate, are an accepted marker of chondrogenic cells. In this study, a cell-based sulfated GAG assay for identifying the chondrogenesis of mesenchymal stem cells was developed. Based on fluorescent staining using safranin O and 4',6-diamidino-2-phenylindole (DAPI), this method was highly sensitive. The results were both qualitative and quantitative. The method is suitable for identifying the chondrogenic process and also for screening compounds. The method may be helpful for discovering novel bioactive compounds for cartilage regeneration.",
keywords = "Chondrogenesis, DAPI, Glycosaminoglycan, Safranin O, Screening",
author = "Yen, {Ching Yu} and Wu, {Yu Wei} and Hsiung, {Chao Nan} and Yeh, {Min I.} and Lin, {Yi Ming} and Lee, {Sheng Yang}",
note = "Funding Information: The study was supported by Grants NSC101-2314-B-038-036-MY2 , NSC102-2314-B-038-017 , and MOST103-2314-B-038-031-MY2 from the National Science Council (Taipei, Taiwan) and Grant 100CM-TMU-11 from Chi Mei Medical Center (Tainan, Taiwan). Appendix A Publisher Copyright: {\textcopyright} 2015 Elsevier Inc. All rights reserved.",
year = "2015",
month = jul,
day = "21",
doi = "10.1016/j.ab.2015.06.005",
language = "English",
volume = "486",
pages = "41--43",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
}