TY - JOUR
T1 - Calcium stimulates mitochondrial biogenesis in human granulosa cells
AU - Tsai, Tian-Shun
AU - Ho, Jau Der
AU - Yang, Vivian Wei Chung
AU - Tzeng, Chii Ruey
AU - Hsieh, Rong Hong
PY - 2005
Y1 - 2005
N2 - Ovarian granulose cells are known to play a key role in regulating ovarian physiology. Age increases apoptosis in follicular granulose cells and subsequently decreases ovarian fecundity. The aging ovary also contains fewer follicles that possess fewer granulose cells. The viability of follicular granulosa cells may be essential for development of the oocyte. Calcium ion plays an important role in a variety of biological processes, including gene expression, cell cycle regulation, and cell death. To study the ability of mitochondrial biogenesis in human granulosa cells, we determined the mitochondrial marker proteins, including the nuclear-encoded NADH-ubiquinone oxidoreductase alpha subunit 9 (NDUFA9) and mitochondrial-encoded COX I, after treatment of the cells with the calcium ionophore A23187. We showed that the expression of these mitochondrial marker proteins in human granulosa cells increased with changes in cytosolic Ca2+ using the ionophore A23187. Treatment of granulosa cells with 0.5 μM of A23187 for 120 h increased the levels of NDUFA 9 and COX I subunit by up to 2.6- and 2.4-fold, respectively. Raising Ca2+ by exposing granulosa cells to 1 μM of A23187 for 48 h significantly increased mitochondrial transcription factor (mtTFA) gene expression by up to 2.9-fold. Our results indicate that the adaptive responses of granulosa cells to increased Ca2+ may include upregulation of mitochondrial proteins and that mtTFA may be involved in such a mitochondrial biogenesis pathway.
AB - Ovarian granulose cells are known to play a key role in regulating ovarian physiology. Age increases apoptosis in follicular granulose cells and subsequently decreases ovarian fecundity. The aging ovary also contains fewer follicles that possess fewer granulose cells. The viability of follicular granulosa cells may be essential for development of the oocyte. Calcium ion plays an important role in a variety of biological processes, including gene expression, cell cycle regulation, and cell death. To study the ability of mitochondrial biogenesis in human granulosa cells, we determined the mitochondrial marker proteins, including the nuclear-encoded NADH-ubiquinone oxidoreductase alpha subunit 9 (NDUFA9) and mitochondrial-encoded COX I, after treatment of the cells with the calcium ionophore A23187. We showed that the expression of these mitochondrial marker proteins in human granulosa cells increased with changes in cytosolic Ca2+ using the ionophore A23187. Treatment of granulosa cells with 0.5 μM of A23187 for 120 h increased the levels of NDUFA 9 and COX I subunit by up to 2.6- and 2.4-fold, respectively. Raising Ca2+ by exposing granulosa cells to 1 μM of A23187 for 48 h significantly increased mitochondrial transcription factor (mtTFA) gene expression by up to 2.9-fold. Our results indicate that the adaptive responses of granulosa cells to increased Ca2+ may include upregulation of mitochondrial proteins and that mtTFA may be involved in such a mitochondrial biogenesis pathway.
KW - Calcium
KW - Granulose cell
KW - Mitochondria
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U2 - 10.1196/annals.1338.017
DO - 10.1196/annals.1338.017
M3 - Article
C2 - 15965058
AN - SCOPUS:22044455181
SN - 0077-8923
VL - 1042
SP - 157
EP - 162
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
ER -