TY - JOUR
T1 - Calcium peroxide aids tyramine-alginate gel to crosslink with tyrosinase for efficient cartilage repair
AU - Wong, Chin Chean
AU - Lu, Chu Xuan
AU - Cho, Er Chen
AU - Lee, Po Wei
AU - Chi, Nai Wen
AU - Lin, Po Yen
AU - Jheng, Pei Ru
AU - Chen, Hsin Lung
AU - Mansel, Bradley W.
AU - Chen, Yu Ming
AU - Chen, Chih Hwa
AU - Chuang, Er Yuan
N1 - Funding Information:
This research work was financially supported by BioGend Therapeutics Co. Ltd. Ministry of Science and Technology of Taiwan (grant nos.: 108-2320-B-038-061-MY3, 108-2221-E-038-017-MY3, and 109-2314-B-038-045-MY3), and Taipei Medical University-Shuang Ho Hospital (110TMU-SHH-22). We appreciate that the Prof. Fwu-Long Mi helps the chemical analysis and provides the ideas of the use of CaO2 on this study.
Funding Information:
This research work was financially supported by BioGend Therapeutics Co., Ltd., Ministry of Science and Technology of Taiwan (grant nos.: 108-2320-B-038-061-MY3 , 108-2221-E-038-017-MY3 , and 109-2314-B-038-045-MY3 ), and Taipei Medical University-Shuang Ho Hospital ( 110TMU-SHH-22 ). We appreciate that the Prof. Fwu-Long Mi helps the chemical analysis and provides the ideas of the use of CaO 2 on this study.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/5/31
Y1 - 2022/5/31
N2 - The innate cartilage extracellular matrix is avascular and plays a vital role in innate chondrocytes. Recapping the crucial components of the extracellular matrix in engineered organs via polymeric gels and bioinspired approaches is promising for improving the regenerative aptitude of encapsulated cartilage/chondrocytes. Conventional gel formation techniques for polymeric materials rely on employing oxidative crosslinking, which is constrained in this avascular environment. Further, poor mechanical properties limit the practical applications of polymeric gels and reduce their therapeutic efficacy. Herein, the purpose of this study was to develop a bioadhesive gel possessing dual crosslinking for engineering cartilage. Tyramine (TYR) was first chemically conjugated to the alginate (ALG) backbone to form an ALG-TYR precursor, followed by the addition of calcium peroxide (CaO2); calcium ions of CaO2 physically crosslink with ALG, and oxygen atoms of CaO2 chemically crosslink TYR with tyrosinase, thus enabling dual/enhanced crosslinking and possessing injectability. The ALG-TYR/tyrosinase/CaO2 gel system was chemically, mechanically, cellularly, and microscopically characterized. The gel system developed herein was biocompatible and showed augmented mechanical strength. The results showed, for the first time, that CaO2 supplementation preserved cell viability and enhanced the crosslinking ability, bioadhesion, mechanical strength, chondrogenesis, and stability for cartilage regeneration.
AB - The innate cartilage extracellular matrix is avascular and plays a vital role in innate chondrocytes. Recapping the crucial components of the extracellular matrix in engineered organs via polymeric gels and bioinspired approaches is promising for improving the regenerative aptitude of encapsulated cartilage/chondrocytes. Conventional gel formation techniques for polymeric materials rely on employing oxidative crosslinking, which is constrained in this avascular environment. Further, poor mechanical properties limit the practical applications of polymeric gels and reduce their therapeutic efficacy. Herein, the purpose of this study was to develop a bioadhesive gel possessing dual crosslinking for engineering cartilage. Tyramine (TYR) was first chemically conjugated to the alginate (ALG) backbone to form an ALG-TYR precursor, followed by the addition of calcium peroxide (CaO2); calcium ions of CaO2 physically crosslink with ALG, and oxygen atoms of CaO2 chemically crosslink TYR with tyrosinase, thus enabling dual/enhanced crosslinking and possessing injectability. The ALG-TYR/tyrosinase/CaO2 gel system was chemically, mechanically, cellularly, and microscopically characterized. The gel system developed herein was biocompatible and showed augmented mechanical strength. The results showed, for the first time, that CaO2 supplementation preserved cell viability and enhanced the crosslinking ability, bioadhesion, mechanical strength, chondrogenesis, and stability for cartilage regeneration.
KW - Calcium peroxide
KW - Cartilage repair
KW - Tyrosinase and alginate-tyramine precursor
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U2 - 10.1016/j.ijbiomac.2022.03.044
DO - 10.1016/j.ijbiomac.2022.03.044
M3 - Article
C2 - 35288166
AN - SCOPUS:85127032893
SN - 0141-8130
VL - 208
SP - 299
EP - 313
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -