@article{66d5cea5e0bc4374ae8cedb1edf97763,
title = "Bone cell responses to a low elastic modulus titanium alloy surface immobilized with the natural cross-linker genipin",
abstract = "In this study, we investigated various responses of human bone marrow mesenchymal stem cells (hMSCs) to a low elastic modulus Ti-24Nb-4Zr-8Sn (Ti2448) alloy surface that underwent immobilization using the natural cross-linker genipin. Specifically, hMSC's adhesion, proliferation, mineralization, and differentiation were evaluated. We also analyzed surface characteristics of the Ti2448 alloy, including chemistry, morphology, roughness, and wettability. Our results suggest that genipin immobilized Ti2448 has the potential to benefit orthopedic and dental implant applications. We found that the immobilization of genipin on a sand-blasted/acid-etched Ti2448 alloy surface significantly enhanced the mineralization of the extracellular matrix as well as the expression of certain osteogenic markers (bone sialoprotein and osteocalcin) in hMSCs. Conversely, surface roughness and hydrophilicity cannot be credited with the improved cellular responses observed in this study. The immobilization of genipin to produce a biomolecular surface on low elastic modulus Ti2448 alloy has considerable potential for bone implant applications.",
keywords = "Cell response, Genipin, Immobilization, Low elastic modulus, Natural crosslinker, Ti alloy",
author = "Sun, {Ying Sui} and Huang, {Chi Ya} and Chen, {Chiang Sang} and Chang, {Jean Heng} and Hou, {Wen Tao} and Li, {Shu Jun} and Hao, {Yu Lin} and Haobo Pan and Huang, {Her Hsiung}",
note = "Funding Information: This study was primarily supported by the Ministry of Science and Technology of Taiwan (MOST 104-2221-E-040-003 and 105-2221-E-040-001 ). This study received partial support from Far Eastern Memorial Hospital (Nos. 105FN20 ; 106DN19 ) and Cheng Hsin General Hospital (Nos. CY10520 ; CY10620 ), Taipei, Taiwan. This study was also partially supported by Chinese MOST ( 2017YFC1104901 and 2016YFC110261 ), Key Research Program of Frontier Sciences, CAS ( QYZDJ-SSW-JSC031-02 ), National Natural Science Foundation of China ( 51591190 , 51771209 , 51631007 ), and Shenzhen Peacock Program ( 110811003586331 ). The authors would like to thank Dr. Shih-Hwa Chiou of Taipei Veterans General Hospital, Taiwan, for providing the human bone marrow mesenchymal stem cells. Funding Information: This study was primarily supported by the Ministry of Science and Technology of Taiwan (MOST 104-2221-E-040-003 and 105-2221-E-040-001). This study received partial support from Far Eastern Memorial Hospital (Nos. 105FN20; 106DN19) and Cheng Hsin General Hospital (Nos. CY10520; CY10620), Taipei, Taiwan. This study was also partially supported by Chinese MOST (2017YFC1104901 and 2016YFC110261), Key Research Program of Frontier Sciences, CAS (QYZDJ-SSW-JSC031-02), National Natural Science Foundation of China (51591190, 51771209, 51631007), and Shenzhen Peacock Program (110811003586331). The authors would like to thank Dr. Shih-Hwa Chiou of Taipei Veterans General Hospital, Taiwan, for providing the human bone marrow mesenchymal stem cells. Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",
year = "2018",
month = sep,
day = "25",
doi = "10.1016/j.surfcoat.2018.03.069",
language = "English",
volume = "350",
pages = "918--924",
journal = "Surface and Coatings Technology",
issn = "0257-8972",
publisher = "Elsevier",
}