@article{922a04e7eb6e4bb5bd91e1ee853cde2a,
title = "Biomaterial-induced conversion of quiescent cardiomyocytes into pacemaker cells in rats",
abstract = "Pacemaker cells can be differentiated from stem cells or transdifferentiated from quiescent mature cardiac cells via genetic manipulation. Here we show that the exposure of rat quiescent ventricular cardiomyocytes to a silk-fibroin hydrogel activates the direct conversion of the quiescent cardiomyocytes to pacemaker cardiomyocytes by inducing the ectopic expression of the vascular endothelial cell-adhesion glycoprotein cadherin. The silk-fibroin-induced pacemaker cells exhibited functional and morphological features of genuine sinoatrial-node cardiomyocytes in vitro, and pacemaker cells generated via the injection of silk fibroin in the left ventricles of rats functioned as a surrogate in situ sinoatrial node. Biomaterials with suitable surface structure, mechanics and biochemistry could facilitate the scalable production of biological pacemakers for human use.",
author = "Hu, {Yu Feng} and Lee, {An Sheng} and Chang, {Shih Lin} and Lin, {Shien Fong} and Weng, {Ching Hui} and Lo, {Hsin Yu} and Chou, {Pei Chun} and Tsai, {Yung Nan} and Sung, {Yen Ling} and Chen, {Chien Chang} and Yang, {Ruey Bing} and Lin, {Yuh Charn} and Kuo, {Terry B.J.} and Wu, {Cheng Han} and Liu, {Jin Dian} and Chung, {Tze Wen} and Chen, {Shih Ann}",
note = "Funding Information: We thank E. Marb?n for reviewing the manuscript, W.-C. Wang for bioinformatics analysis, W.-H. Hsu for technical assistance, S.-C. Chou for flow cytometry analysis and the MiaoLi District Agriculture Research and Extension Station (Council of Agriculture, MiaoLi, Taiwan) for providing cocoons, which are also available for experimental reproduction by others. This work was supported by the Taipei Veterans General Hospital (V107C-029, VGHUST-107-G5-1-2, VGH108C-019, VN108-12, V110C-039), the Ministry of Science and Technology (105-2633-B-075-002, 107-2628-B-075-003, 108-2628-B-075-003, 107-2221-E-010-005-MY3, 110-2628-B-075-015), the National Health Research Institutes (NHRI-EX108-10513SC) and Academia Sinica (AS-TM-109-01-05, AS-TM-110-01-01). We also thank the National RNAi Core Facility at Academia Sinica in Taiwan for providing services, and C.-M. J. Hu for providing PEG-DA. Funding Information: We thank E. Marb{\'a}n for reviewing the manuscript, W.-C. Wang for bioinformatics analysis, W.-H. Hsu for technical assistance, S.-C. Chou for flow cytometry analysis and the MiaoLi District Agriculture Research and Extension Station (Council of Agriculture, MiaoLi, Taiwan) for providing cocoons, which are also available for experimental reproduction by others. This work was supported by the Taipei Veterans General Hospital (V107C-029, VGHUST-107-G5-1-2, VGH108C-019, VN108-12, V110C-039), the Ministry of Science and Technology (105-2633-B-075-002, 107-2628-B-075-003, 108-2628-B-075-003, 107-2221-E-010-005-MY3, 110-2628-B-075-015), the National Health Research Institutes (NHRI-EX108-10513SC) and Academia Sinica (AS-TM-109-01-05, AS-TM-110-01-01). We also thank the National RNAi Core Facility at Academia Sinica in Taiwan for providing services, and C.-M. J. Hu for providing PEG-DA. Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to Springer Nature Limited.",
year = "2022",
month = apr,
doi = "10.1038/s41551-021-00812-y",
language = "English",
volume = "6",
pages = "421--434",
journal = "Nature Biomedical Engineering",
issn = "2157-846X",
publisher = "Nature Publishing Group",
number = "4",
}