TY - CHAP
T1 - Biogenesis of Mesoporous Silica Nanoparticles Enclosed in Extracellular Vesicles by Mouse Renal Adenocarcinoma Cells
AU - Pratiwi, Feby Wijaya
AU - Shanthi, Keerthanaa Balasubramanian
AU - Makieieva, Olha
AU - Chen, Zih An
AU - Zhyvolozhnyi, Artem
AU - Miinalainen, Ilkka
AU - Bart, Genevieve
AU - Samoylenko, Anatoliy
AU - Wu, Si Han
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - Integrating the versatility of synthetic nanoparticles to natural biomaterials, such as cells or cell membranes, has gained considerable attention as promising alternative cargo delivery platforms in recent years. Extracellular vesicles (EVs), natural nanomaterials composed of a protein-rich lipid bilayer secreted by cells, have also shown advantages and great potential as a nano delivery platform in combination with synthetic particles due to their specific natural properties in overcoming several biology hurdles possessed in the recipient cell. Therefore, the preservation of EV’s origin properties is critical for their application as nanocarriers. This chapter will describe the encapsulation procedure of MSN encapsulated in EV membrane derived from mouse renal adenocarcinoma (Renca) cells through biogenesis. The FMSN-enclosed EVs produced through this approach still contain preserved EV’s natural membrane properties.
AB - Integrating the versatility of synthetic nanoparticles to natural biomaterials, such as cells or cell membranes, has gained considerable attention as promising alternative cargo delivery platforms in recent years. Extracellular vesicles (EVs), natural nanomaterials composed of a protein-rich lipid bilayer secreted by cells, have also shown advantages and great potential as a nano delivery platform in combination with synthetic particles due to their specific natural properties in overcoming several biology hurdles possessed in the recipient cell. Therefore, the preservation of EV’s origin properties is critical for their application as nanocarriers. This chapter will describe the encapsulation procedure of MSN encapsulated in EV membrane derived from mouse renal adenocarcinoma (Renca) cells through biogenesis. The FMSN-enclosed EVs produced through this approach still contain preserved EV’s natural membrane properties.
KW - Biogenesis
KW - Extracellular vesicle
KW - Hybrid nanoparticles
KW - Mesoporous silica nanoparticle
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U2 - 10.1007/978-1-0716-3203-1_17
DO - 10.1007/978-1-0716-3203-1_17
M3 - Chapter
C2 - 37140801
AN - SCOPUS:85159144936
T3 - Methods in Molecular Biology
SP - 241
EP - 256
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -