Bacteriophage-linked superparamagnetic nanoparticle for sensitive and simple detection of pathogenic Escherichia coli O157:H7 bacteria

Sheng Jie Shiue, Ming Shun Wu, Jen Yu Liao, Wei Cheng Sun, Hsin Yi Lin

研究成果: 雜誌貢獻文章同行評審

摘要

An easy-to-use and highly-sensitive magnetic nanoprobe to detect veratoxin-producing Escherichia coli O157:H7 was developed. The bacteriophage that targets E. coli O157:H7 was chemically linked to chitosan-coated iron oxide nanoparticles (5–50 nm) to form a nanoprobe for the detection of E. coli O157:H7 in aqueous samples. The nanoprobes had a limit of detection of 2 log CFU/mL in samples with pH from 5 to 8 at 25 °C, and temperatures from 25 to 40 °C at pH 7. In the beef and cabbage liquid extracts, the nanoprobe had a detection limit of 2 log CFU/mL at 25 °C and pH 7. The nanoprobe was highly specific and retrieved only E. coli O157:H7 from a sample of bacterial mixture. Gram staining method was used with the nanoprobe to visualize the bacterial cells captured from bacterial suspensions at 7 pH and 25 °C. Visualization of the bacterial cells captured from suspensions with a concentration of 2 log CFU/mL was achieved. To detect the presence of bacteria, the use of the magnetic nanoprobes and Gram staining took less than 30 min to complete. The system developed in this study offers a fast and operator-friendly alternative for highly-specific bacterial detection.
原文英語
文章編號111896
期刊Microchemical Journal
207
DOIs
出版狀態已發佈 - 12月 2024

ASJC Scopus subject areas

  • 分析化學
  • 光譜

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