Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V

Kohei Murata; Eiji Miyoshi; Shinji Ihara; Shingo Noura; Masao Kameyama; Osamu Ishikawa; Yuichiro Doki; Terumasa Yamada; Hiroaki Ohigashi; Yo Sasaki; Masahiko Higashiyama; Takehiko Tarui; Yoshikazu Takada; Reiji Kannagi; Naoyuki Taniguchi; Shingi Imaoka

doi:10.1159/000079504

Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V

Kohei Murata, Eiji Miyoshi, Shinji Ihara, Shingo Noura, Masao Kameyama, Osamu Ishikawa, Yuichiro Doki, Terumasa Yamada, Hiroaki Ohigashi, Yo Sasaki, Masahiko Higashiyama, Takehiko Tarui, Yoshikazu Takada, Reiji Kannagi, Naoyuki Taniguchi, Shingi Imaoka

研究成果: 雜誌貢獻 › 文章 › 同行評審

32 引文斯高帕斯（Scopus）

摘要

Expression of N-acetylglucosaminyltransferase V (GnT-V) in colon cancer has been shown to be related to hematogenous metastasis and poor prognosis. To investigate the mechanism by which cancer cells expressing GnT-V metastasize to distant organs, we established GnT-Voverexpressing DLD-1 and WiDr cells (human colon cancer cell lines) by transfecting them with a GnT-V expression vector. Attachment to endothelial cells expressing E-selectin was studied, and expression of the E-selectin ligand, sialyl Lewis x, in colon cancer cells was investigated. Both of the cell lines showed reduced adhesion to fibronectin as compared with mock transfectants. In contrast, attachment to human umbilical vein endothelial cells expressing E-selectin was significantly enhanced by GnT-V expression (p

原文	英語
頁（從 - 到）	492-501
頁數	10
期刊	Oncology
卷	66
發行號	6
DOIs	https://doi.org/10.1159/000079504
出版狀態	已發佈 - 2004
對外發佈	是

ASJC Scopus subject areas

癌症研究
腫瘤科

UN SDG

此研究成果有助於以下永續發展目標

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10.1159/000079504

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Murata, K., Miyoshi, E., Ihara, S., Noura, S., Kameyama, M., Ishikawa, O., Doki, Y., Yamada, T., Ohigashi, H., Sasaki, Y., Higashiyama, M., Tarui, T., Takada, Y., Kannagi, R., Taniguchi, N., & Imaoka, S. (2004). Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V. Oncology, 66(6), 492-501. https://doi.org/10.1159/000079504

Murata, K, Miyoshi, E, Ihara, S, Noura, S, Kameyama, M, Ishikawa, O, Doki, Y, Yamada, T, Ohigashi, H, Sasaki, Y, Higashiyama, M, Tarui, T, Takada, Y, Kannagi, R, Taniguchi, N & Imaoka, S 2004, 'Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V', Oncology, 卷 66, 編號 6, 頁 492-501. https://doi.org/10.1159/000079504

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title = "Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V",

abstract = "Expression of N-acetylglucosaminyltransferase V (GnT-V) in colon cancer has been shown to be related to hematogenous metastasis and poor prognosis. To investigate the mechanism by which cancer cells expressing GnT-V metastasize to distant organs, we established GnT-Voverexpressing DLD-1 and WiDr cells (human colon cancer cell lines) by transfecting them with a GnT-V expression vector. Attachment to endothelial cells expressing E-selectin was studied, and expression of the E-selectin ligand, sialyl Lewis x, in colon cancer cells was investigated. Both of the cell lines showed reduced adhesion to fibronectin as compared with mock transfectants. In contrast, attachment to human umbilical vein endothelial cells expressing E-selectin was significantly enhanced by GnT-V expression (p ",

keywords = "Colon cancer, E-Selectin, Mannoside acetylglucosaminyl transferase 5, N-acetylglucosaminyltransferase V, Sialyl Lewis x",

author = "Kohei Murata and Eiji Miyoshi and Shinji Ihara and Shingo Noura and Masao Kameyama and Osamu Ishikawa and Yuichiro Doki and Terumasa Yamada and Hiroaki Ohigashi and Yo Sasaki and Masahiko Higashiyama and Takehiko Tarui and Yoshikazu Takada and Reiji Kannagi and Naoyuki Taniguchi and Shingi Imaoka",

year = "2004",

doi = "10.1159/000079504",

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pages = "492--501",

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T1 - Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V

AU - Murata, Kohei

AU - Miyoshi, Eiji

AU - Ihara, Shinji

AU - Noura, Shingo

AU - Kameyama, Masao

AU - Ishikawa, Osamu

AU - Doki, Yuichiro

AU - Yamada, Terumasa

AU - Ohigashi, Hiroaki

AU - Sasaki, Yo

AU - Higashiyama, Masahiko

AU - Tarui, Takehiko

AU - Takada, Yoshikazu

AU - Kannagi, Reiji

AU - Taniguchi, Naoyuki

AU - Imaoka, Shingi

PY - 2004

Y1 - 2004

N2 - Expression of N-acetylglucosaminyltransferase V (GnT-V) in colon cancer has been shown to be related to hematogenous metastasis and poor prognosis. To investigate the mechanism by which cancer cells expressing GnT-V metastasize to distant organs, we established GnT-Voverexpressing DLD-1 and WiDr cells (human colon cancer cell lines) by transfecting them with a GnT-V expression vector. Attachment to endothelial cells expressing E-selectin was studied, and expression of the E-selectin ligand, sialyl Lewis x, in colon cancer cells was investigated. Both of the cell lines showed reduced adhesion to fibronectin as compared with mock transfectants. In contrast, attachment to human umbilical vein endothelial cells expressing E-selectin was significantly enhanced by GnT-V expression (p

AB - Expression of N-acetylglucosaminyltransferase V (GnT-V) in colon cancer has been shown to be related to hematogenous metastasis and poor prognosis. To investigate the mechanism by which cancer cells expressing GnT-V metastasize to distant organs, we established GnT-Voverexpressing DLD-1 and WiDr cells (human colon cancer cell lines) by transfecting them with a GnT-V expression vector. Attachment to endothelial cells expressing E-selectin was studied, and expression of the E-selectin ligand, sialyl Lewis x, in colon cancer cells was investigated. Both of the cell lines showed reduced adhesion to fibronectin as compared with mock transfectants. In contrast, attachment to human umbilical vein endothelial cells expressing E-selectin was significantly enhanced by GnT-V expression (p

KW - Colon cancer

KW - E-Selectin

KW - Mannoside acetylglucosaminyl transferase 5

KW - N-acetylglucosaminyltransferase V

KW - Sialyl Lewis x

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Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V

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UN SDG

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