Association of globular β-actin with intracellular lipid droplets in rat adrenocortical cells and adipocytes

Tsorng Harn Fong, Ching Hsiang Wu, E. Wen Liao, Chiu Yun Chang, Man Hui Pai, Ruei Jen Chiou, Ai Wei Lee

研究成果: 雜誌貢獻文章同行評審

15 引文 斯高帕斯(Scopus)

摘要

Proteins located on the surface of lipid droplets may mediate intracellular lipid metabolism. In the present study, immunofluorescent staining and polyacrylamide gel electrophoresis demonstrated that actin (43 kD) is associated with isolated intracellular lipid droplets of rat adrenocortical cells and adipocytes. Two-dimensional gel electrophoresis and immunoblot analysis further confirmed that the lipid droplet-associated actin is the beta isoform. In cultured adrenocortical cells, stress fibers and the surface of intracellular lipid droplets were labeled with anti-beta-actin monoclonal antibody, whereas FITC-phalloidin staining did not mark the rim of lipid droplets. The present results provide the first morphological evidence that globular beta-actin is associated with intracellular lipid droplets. This significant association of actin with the surface of lipid droplets suggests that beta-actin might be involved in the regulation of intracellular lipid metabolism, particularly providing insight into the important transport of lipid constituents.

原文英語
頁(從 - 到)1168-1174
頁數7
期刊Biochemical and Biophysical Research Communications
289
發行號5
DOIs
出版狀態已發佈 - 12月 21 2001

ASJC Scopus subject areas

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學

指紋

深入研究「Association of globular β-actin with intracellular lipid droplets in rat adrenocortical cells and adipocytes」主題。共同形成了獨特的指紋。

引用此