Assessing cardiovascular toxicity in zebrafish embryos exposed to copper nanoparticles

Bu Yuan Hsiao, Jiun Lin Horng, Ching Hua Yu, Wen Ting Lin, Yu Han Wang, Li Yih Lin

研究成果: 雜誌貢獻文章同行評審


The toxicity of copper nanoparticles (CuNPs) to aquatic animals, particularly their effects on the cardiovascular system, has not been thoroughly investigated. In the present study, zebrafish embryos were used as a model to address this issue. After exposure to different concentrations (0.01, 0.1, 1, and 3 mg/L) of CuNPs for 96 h (4 to 100 h post-fertilization), cardiac parameters of the heart rate (HR), end-diastolic volume (EDV), end-systolic volume (ESV), stroke volume (SV), ejection fraction (EF), and cardiac output (CO), and vascular parameters of the aortic blood flow velocity (ABFV) and aortic diameter (AD) were examined by a video-microscopic method. Morphologically, CuNPs induced concentration-dependent pericardial edema. Although CuNPs did not alter the HR, they significantly reduced the EDV, SV, and CO at ≥0.1 mg/L, the ESV and EF at 3 mg/L, the ABFV at ≥0.1 mg/L, and the AD at ≥1 mg/L. Transcript levels of several cardiac genes, nppa, nppb, vmhc, and gata4, were also examined. CuNPs significantly suppressed nppa and nppb at ≥0.1 mg/L, gata4 at ≥0.01 mg/L, and vmhc at 1 mg/L. This study demonstrated that CuNPs can induce cardiovascular toxicity at environmentally relevant concentrations during fish embryonic development and highlight the potential ecotoxicity of CuNPs to aquatic animals.
期刊Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
出版狀態已發佈 - 3月 2024

ASJC Scopus subject areas

  • 生物化學
  • 生理學
  • 海洋科學
  • 動物科學與動物學
  • 毒理學
  • 細胞生物學
  • 健康、毒理學和誘變


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