@article{42fd3dc17f3d48b4a8bea7ae851da68f,
title = "Arecoline-regulated ataxia telangiectasia mutated expression level in oral cancer progression",
abstract = "Background: Ataxia telangiectasia mutated (ATM) regulates DNA repair and cell cycle. The present study analyzed arecoline-induced ATM expression during oral cancer progression. Methods: In vitro studies were performed using oral squamous cell carcinoma (OSCC) cell lines treated with arecoline to analyze cell response and ATM regulation. in vivo studies were performed using immunohistochemistry to detect ATM expression in normal, oral potentially malignant disorder (OPMD), and OSCC tissues. Results: Low-dose arecoline induced cell proliferation, ATM promoter activity, and DNA repair. High-dose arecoline induced cell cycle arrest, apoptosis, and DNA damage. ATM was overexpressed in OPMD tissues but was downregulated in OSCC tissues. ATM expression level was associated with the risk of developing dysplasia, buccal-OSCC, and with OSCC survival rate. Conclusion: High ATM expression helps DNA repair mechanisms to maintain the cells in the OPMD stage, but low ATM expression causes DNA damage accumulation to increase cell malignancy.",
keywords = "arecoline, ataxia telangiectasia mutated (ATM), DNA repair, oral potentially malignant disorder (OPMD), oral squamous cell carcinoma (OSCC)",
author = "Tu, {Hsi Feng} and Chen, {Michael Yuanchien} and Lai, {Joseph Chieh Yui} and Chen, {Yi Ling} and Wong, {Yih Wen} and Yang, {Cheng Chieh} and Chen, {Hsin Yuan} and Hsia, {Shih Min} and Shih, {Yin Hwa} and Shieh, {Tzong Ming}",
note = "Funding Information: The authors thank Mr. Chiu, Peng-Chih for the immunohistochemistry-related experiments in this study. This study was supported by grants from China Medical University (CMU103-S-38, CMU107-S-37) and the Ministry of Science and Technology, R.O.C. (104-2815-C-039-041-B, MOST 106-2314-B-039-003-MY2). Funding Information: information China Medical University, Grant/Award Numbers: CMU103-S-38, CMU107-S-37; Ministry of Science and Technology, R.O.C., Grant/Award Numbers: 104-2815-C-039-041-B, MOST 106-2314-B-039-003-MY2 The authors thank Mr. Chiu, Peng-Chih for the immunohistochemistry-related experiments in this study. This study was supported by grants from China Medical University (CMU103-S-38, CMU107-S-37) and the Ministry of Science and Technology, R.O.C. (104-2815-C-039-041-B, MOST 106-2314-B-039-003-MY2). Publisher Copyright: {\textcopyright} 2019 Wiley Periodicals, Inc.",
year = "2019",
month = aug,
doi = "10.1002/hed.25718",
language = "English",
volume = "41",
pages = "2525--2537",
journal = "Head and Neck",
issn = "1043-3074",
publisher = "John Wiley and Sons Inc.",
number = "8",
}
