Antioxidant effects of ginsenoside Re in cardiomyocytes

Jing Tian Xie, Zuo Hui Shao, Terry L. Vanden Hoek, Wei Tien Chang, Jing Li, Sangeeta Mehendale, Chong Zhi Wang, Chin Wang Hsu, Lance B. Becker, Jun Jie Yin, Chun Su Yuan

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165 引文 斯高帕斯(Scopus)


We have previously demonstrated that American ginseng berry extract exhibited significant protection against oxidant-mediated injury in cardiomyocytes. To extend this work, we sought to investigate the antioxidant effects of Re, a protopanaxatriols-type and single chemical integrant present in American ginseng berry extract, using the same chick cardiomyocyte model of oxidant injury as well as ESR spectroscopy in a cell-free chemical system. In cells exposed to 2 h of H2O2 (0.5 mM), pretreatment with Re (0.05, 0.1, or 0.5 mg/ml for 2 h) significantly attenuated 2′,7′-dichlorofluorescein (DCF) fluorescence by 51% (from 1345 ± 67 to 658 ± 46 a.u., P < 0.001), and remarkably reduced cell death (from 51.5 ± 3.0% to 11.8 ± 1.5%, P < 0.001, compared to the control). Similar results were also observed in cells exposed to antimycin A (100 μM), a mitochondrial electron transport chain site III inhibitor which increases endogenous oxidative stress. In the ESR study, however, Re failed to reduce the formation of the superoxide/DMPO adduct and DPPH radicals. These results suggest that ginsenoside Re functions as an antioxidant, protecting cardiomyocytes from oxidant injury induced by both exogenous and endogenous oxidants, and that its protective effects may be mostly attributed to scavenging H2O2 and hydroxyl radicals.

頁(從 - 到)201-207
期刊European Journal of Pharmacology
出版狀態已發佈 - 2月 27 2006

ASJC Scopus subject areas

  • 藥理


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