Antioral Cancer Effects by the Nitrated [6,6,6]Tricycles Compound (SK1) In Vitro

Yan Ning Chen, Chieh Kai Chan, Ching Yu Yen, Jun Ping Shiau, Meng Yang Chang, Cheng Chung Wang, Jiiang Huei Jeng, Jen Yang Tang, Hsueh Wei Chang

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)


A novel nitrated [6,6,6]tricycles-derived compound containing nitro, methoxy, and ispropyloxy groups, namely SK1, was developed in our previous report. However, the anticancer effects of SK1 were not assessed. Moreover, SK1 contains two nitro groups (NO2) and one nitrogen-oxygen (N-O) bond exhibiting the potential for oxidative stress generation, but this was not examined. The present study aimed to evaluate the antiproliferation effects and oxidative stress and its associated responses between oral cancer and normal cells. Based on the MTS assay, SK1 demonstrated more antiproliferation ability in oral cancer cells than normal cells, reversed by N-acetylcysteine. This suggests that SK1 causes antiproliferation effects preferentially in an oxidative stress-dependent manner. The oxidative stress-associated responses were further validated, showing higher ROS/MitoSOX burst, MMP, and GSH depletion in oral cancer cells than in normal cells. Meanwhile, SK1 caused oxidative stress-causing apoptosis, such as caspases 3/8/9, and DNA damages, such as γH2AX and 8-OHdG, to a greater extent in oral cancer cells than in normal cells. Siilar to cell viability, these oxidative stress responses were partially diminished by NAC, indicating that SK1 promoted oxidative stress-dependent responses. In conclusion, SK1 exerts oxidative stress, apoptosis, and DNA damage to a greater extent to oral cancer cells than in normal cells.
出版狀態已發佈 - 10月 2022

ASJC Scopus subject areas

  • 食品科學
  • 生理學
  • 生物化學
  • 分子生物學
  • 臨床生物化學
  • 細胞生物學


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