Antibacterial effect of the natural polymer "-Polylysine against oral pathogens associated with periodontitis and caries

Shinechimeg Dima, Yin Yin Lee, Ikki Watanabe, Wei Jen Chang, Yu Hua Pan, Nai Chia Teng

研究成果: 雜誌貢獻文章同行評審

15 引文 斯高帕斯(Scopus)


Antimicrobials are important adjuncts in the treatment of caries and periodontitis. However, increased bacterial resistance and hypersensitivity reactions to commonly used antimicrobials have led to an increasing demand for safe and natural substances. The objective of this study was to investigate the antibacterial effects of "-polylysine against oral pathogens Streptococcus mutans and Porphyromonas gingivalis. Broth dilution assay, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) analyses were performed to explore the antibacterial effect of "-polylysine against S. mutans strain ATCC25175 and P. gingivalis strain ATCC332277. For the test solution, "-polylysine was added to the bacterial suspension to prepare 0.125%, 0.25%, 0.5% and 1% "-polylysine solutions diluted in broth medium. All four concentrations demonstrated complete inhibition of S. mutans and significantly reduced viable cell counts of P. gingivalis after 24 h. From starting inoculum of 9.15 log CFU/mL, P. gingivalis cell counts reduced to 4.01 log CFU/mL in the 0.125% "-polylysine treatment group. SEM, CLSM, and the LIVE/DEAD bacterial assay of "-polylysine application on P. gingivalis biofilm-dentin specimens revealed bacterial cell membrane disruption and irregular cell morphologies. The results indicated satisfactory antibacterial efficacy of "-polylysine against P. gingivalis and S. mutans in liquid medium and as an application on biofilm-dentin specimens.

出版狀態已發佈 - 6月 1 2020

ASJC Scopus subject areas

  • 一般化學
  • 聚合物和塑料


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