Anti-proliferative and gene expression actions of resveratrol in breast cancer cells in vitro

Yu Tang Chin, Meng Ti Hsieh, Sheng Huei Yang, Po Wei Tsai, Shwu Huey Wang, Ching Chiung Wang, Yee Shin Lee, Guei Yun Cheng, Wei Chun HuangFu, David London, Heng Yuan Tang, Earl Fu, Yun Yen, Leroy F. Liu, Hung Yun Lin, Paul J. Davis

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65 引文 斯高帕斯(Scopus)


We have used a perfusion bellows cell culture system to investigate resveratrolinduced anti-proliferation/apoptosis in a human estrogen receptor (ER)-negative breast cancer cell line (MDA-MB-231). Using an injection system to perfuse media with stilbene, we showed resveratrol (0.5 - 100 μM) to decrease cell proliferation in a concentration-dependent manner. Comparison of influx and medium efflux resveratrol concentrations revealed rapid disappearance of the stilbene, consistent with cell uptake and metabolism of the agent reported by others. Exposure of cells to 10 μM resveratrol for 4 h daily × 6 d inhibited cell proliferation by more than 60%. Variable extracellular acid-alkaline conditions (pH 6.8 - 8.6) affected basal cell proliferation rate, but did not alter anti-proliferation induced by resveratrol. Resveratrol-induced gene expression, including transcription of the most up-regulated genes and proapoptotic p53-dependent genes, was not affected by culture pH changes. The microarray findings in the context of induction of anti-proliferation with brief daily exposure of cells to resveratrol-and rapid disappearance of the compound in the perfusion system-are consistent with existence of an accessible initiation site for resveratrol actions on tumor cells, e.g., the cell surface receptor for resveratrol described on integrin αvβ3.
頁(從 - 到)12891-12907
出版狀態已發佈 - 2014

ASJC Scopus subject areas

  • 腫瘤科


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