@article{d96b7b14bbeb4d348eea577cb0d44348,
title = "Anti-inflammatory and antiviral activities of flavone C-glycosides of Lophatherum gracile for COVID-19",
abstract = "Lophatherum gracile (L. gracile) has long been used as a functional food and herbal medicine. Previous studies have demonstrated that extracts of L. gracile attenuate inflammatory response and inhibit SARS-CoV-2 replication; however, the underlying active constituents have yet to be identified. This study investigated the bioactive components of L. gracile. Flavone C-glycosides of L. gracile were found to dominate both anti-inflammatory and antiviral effects. A simple chromatography-based method was developed to obtain flavone C-glycoside-enriched extract (FlavoLG) from L. gracile. FlavoLG and its major flavone C-glycoside isoorientin were shown to restrict respiratory bursts and the formation of neutrophil extracellular traps in activated human neutrophils. FlavoLG and isoorientin were also shown to inhibit SARS-CoV-2 pseudovirus infection by interfering with the binding of the SARS-CoV-2 spike on ACE2. These results provide scientific evidence indicating the efficacy of L. gracile as a potential supplement for treating neutrophil-associated COVID-19.",
keywords = "COVID-19, Flavone C-glycosides, Lophatherum gracile, Neutrophils, SARS-CoV-2",
author = "Chen, {Yu Li} and Chen, {Chun Yu} and Lai, {Kuei Hung} and Chang, {Yu Chia} and Hwang, {Tsong Long}",
note = "Funding Information: This work was supported by grants from the Chang Gung Memorial Hospital (CMRPG3K0921, CMRPF1L0071, CMRPF1M0101, CMRPF1M0131, CMRPF1M0091 and CORPF1L0011), the Chang Gung University of Science and Technology (ZRRPF3L0091), and the National Science and Technology Council (108–2320-B-255–003-MY3, 109–2811-B-255–502, 109–2327-B-255–001, 111–2321-B-255–001, 111–2320-B-255–006-MY3, 111–2811-B-255–002, 111–2321-B-182–001). Funding Information: We would like to thank the National RNAi Core Facility at Academia Sinica in Taiwan for providing the SARS-CoV-2 pseudovirus and related services. We thank Prof. Rei-Lin Kuo (Chang Gung University) for kindly providing hACE-2-overexpressed HEK293T cells. Publisher Copyright: {\textcopyright} 2023 The Authors",
year = "2023",
month = feb,
doi = "10.1016/j.jff.2023.105407",
language = "English",
volume = "101",
journal = "Journal of Functional Foods",
issn = "1756-4646",
publisher = "Elsevier Ltd",
}