TY - JOUR
T1 - Anti-inflammatory activity of mangostins from Garcinia mangostana
AU - Chen, Lih Geeng
AU - Yang, Ling-Ling
AU - Wang, Ching Chiung
PY - 2008/2
Y1 - 2008/2
N2 - The fruit hull of Garcinia mangostana Linn (Guttiferae) is used as an anti-inflammatory drug in Southeast Asia. Two xanthones, α- and γ-mangostins, were isolated from the fruit hull of G. mangostana, and both significantly inhibited nitric oxide (NO) and PGE2 production from lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The IC50 values for the inhibition of NO production by α- and γ-mangostins were 12.4 and 10.1 μM, respectively. After iNOS enzyme activity was stimulated by LPS for 12 h, treatment with either α- or γ-mangostin at 5 μg/ml (12.2 and 12.6 μM, respectively) for 24 h did not significantly inhibit NO production. The data show that the inhibitory activities of α- and γ-mangostins are not due to direct inhibition of iNOS enzyme activity. On the other hand, expression of iNOS was inhibited by α- and γ-mangostins in LPS-stimulated RAW 264.7 cells, but not by COX-2. However, the level of PGE2 production was reduced by the two xanthones. In an in vivo study, α-mangostin significantly inhibited mice carrageenan-induced paw edema. In conclusion, α- and γ-mangostins from G. mangostana are bioactive substances with anti-inflammatory effects.
AB - The fruit hull of Garcinia mangostana Linn (Guttiferae) is used as an anti-inflammatory drug in Southeast Asia. Two xanthones, α- and γ-mangostins, were isolated from the fruit hull of G. mangostana, and both significantly inhibited nitric oxide (NO) and PGE2 production from lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The IC50 values for the inhibition of NO production by α- and γ-mangostins were 12.4 and 10.1 μM, respectively. After iNOS enzyme activity was stimulated by LPS for 12 h, treatment with either α- or γ-mangostin at 5 μg/ml (12.2 and 12.6 μM, respectively) for 24 h did not significantly inhibit NO production. The data show that the inhibitory activities of α- and γ-mangostins are not due to direct inhibition of iNOS enzyme activity. On the other hand, expression of iNOS was inhibited by α- and γ-mangostins in LPS-stimulated RAW 264.7 cells, but not by COX-2. However, the level of PGE2 production was reduced by the two xanthones. In an in vivo study, α-mangostin significantly inhibited mice carrageenan-induced paw edema. In conclusion, α- and γ-mangostins from G. mangostana are bioactive substances with anti-inflammatory effects.
KW - α- and γ-mangostins
KW - COX-2
KW - Garcinia mangostana Linn
KW - Guttiferae
KW - Inducible nitric oxide synthase
KW - RAW 264.7 murine macrophages
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U2 - 10.1016/j.fct.2007.09.096
DO - 10.1016/j.fct.2007.09.096
M3 - Article
C2 - 18029076
AN - SCOPUS:37349007803
SN - 0278-6915
VL - 46
SP - 688
EP - 693
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 2
ER -