TY - JOUR
T1 - An androgen-regulated miRNA suppresses Bak1 expression and induces androgen-independent growth of prostate cancer cells
AU - Shi, Xu Bao
AU - Xue, Lingru
AU - Yang, Joy
AU - Ma, Ai Hong
AU - Zhao, Jianjun
AU - Xu, Ma
AU - Tepper, Clifford G.
AU - Evans, Christopher P.
AU - Kung, Hsing Jien
AU - White, Ralph W DeVere
PY - 2007/12/11
Y1 - 2007/12/11
N2 - Although prostate cancer (CaP) is the most frequently diagnosed malignant tumor and the second leading cause of cancer deaths in American men, the mechanisms explaining the development and progression of CaP remain largely unknown. Recent studies have shown that some aberrantly expressed microRNAs (miRNAs) are involved in tumorigenesis. Although aberrant expression of certain miRNAs has been discovered in CaP, their function in this disease has not yet been defined. In this study, we found differential expression of miR-125b in androgen-dependent and independent CaP cells, as well as in benign and malignant prostate tissues. Furthermore, androgen signaling was able to up-regulate the expression of miR-125b. In addition, transfection of synthetic miR-125b stimulated androgen-independent growth of CaP cells and down-regulated the expression of Bak1. Our results suggest that miR-125b acts as an oncogene, contributing to the pathogenesis of CaP.
AB - Although prostate cancer (CaP) is the most frequently diagnosed malignant tumor and the second leading cause of cancer deaths in American men, the mechanisms explaining the development and progression of CaP remain largely unknown. Recent studies have shown that some aberrantly expressed microRNAs (miRNAs) are involved in tumorigenesis. Although aberrant expression of certain miRNAs has been discovered in CaP, their function in this disease has not yet been defined. In this study, we found differential expression of miR-125b in androgen-dependent and independent CaP cells, as well as in benign and malignant prostate tissues. Furthermore, androgen signaling was able to up-regulate the expression of miR-125b. In addition, transfection of synthetic miR-125b stimulated androgen-independent growth of CaP cells and down-regulated the expression of Bak1. Our results suggest that miR-125b acts as an oncogene, contributing to the pathogenesis of CaP.
KW - ISH
KW - LNCaP
KW - microRNA
KW - miR-125b
UR - http://www.scopus.com/inward/record.url?scp=38049100559&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38049100559&partnerID=8YFLogxK
U2 - 10.1073/pnas.0706641104
DO - 10.1073/pnas.0706641104
M3 - Article
C2 - 18056640
AN - SCOPUS:38049100559
SN - 0027-8424
VL - 104
SP - 19983
EP - 19988
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 50
ER -
