摘要
Angiotensin II (Ang II) is a risk factor for cardiovascular disease. We used a traditional Chinese medicine, alpinate oxyphyllae fructus (AOF), to evaluate its effect on Ang II-induced cardiac apoptosis and mitochondrial dysfunction. Ang II-treated H9c2 cells were administered AOF of 20-100μg/mL concentrations. Ang II significantly increased TUNEL-positive nuclei in the H9c2 cells, effect was inhibited by AOF administration in both pre-treated and post-treated H9c2 cells. Caspases 9 and 3 activities were increased by Ang II and downregulated by AOF administration, especially in pre-treatment. AOF treatment reversed Ang II-induced mitochondria membrane potential instability in H9c2 cells as observed by JC-1 stain assay. Furthermore, proapoptotic proteins Bad and cytochrome c increased and decreased respectively under AOF administration. The levels of p-Bad anti-apoptotic protein were significantly increased after AOF treatment. This study indicates that mitochondrial dependent apoptosis induced by Ang II.
原文 | 英語 |
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頁(從 - 到) | 229-234 |
頁數 | 6 |
期刊 | Bioscience, Biotechnology and Biochemistry |
卷 | 77 |
發行號 | 2 |
DOIs | |
出版狀態 | 已發佈 - 2013 |
ASJC Scopus subject areas
- 生物技術
- 生物化學
- 分子生物學
- 應用微生物與生物技術
- 分析化學
- 有機化學