Aldehyde dehydrogenase 1 is a putative marker for cancer stem cells in head and neck squamous cancer

Yu Chih Chen; Yi Wei Chen; Han Shui Hsu; Ling Ming Tseng; Pin I. Huang; Kai Hsi Lu; Dow Tien Chen; Lung Kuo Tai; Ming Chi Yung; Shih Ching Chang; Hung Hai Ku; Shih Hwa Chiou; Wen Liang Lo

doi:10.1016/j.bbrc.2009.05.048

Aldehyde dehydrogenase 1 is a putative marker for cancer stem cells in head and neck squamous cancer

Yu Chih Chen, Yi Wei Chen, Han Shui Hsu, Ling Ming Tseng, Pin I. Huang, Kai Hsi Lu, Dow Tien Chen, Lung Kuo Tai, Ming Chi Yung, Shih Ching Chang, Hung Hai Ku, Shih Hwa Chiou, Wen Liang Lo

研究成果: 雜誌貢獻 › 文章 › 同行評審

424 引文斯高帕斯（Scopus）

摘要

Aldehyde dehydrogenase 1 (ALDH1) has been considered to be a marker for cancer stem cells. However, the role of ALDH1 in head and neck squamous cell carcinoma (HNSCC) has yet to be determined. In this study, we isolated ALDH1-positive cells from HNSCC patients and showed that these HNSCC-ALDH1⁺ cells displayed radioresistance and represented a reservoir for generating tumors. Based on microarray findings, the results of Western blotting and immunofluorescent assays further confirmed that ALDH1⁺-lineage cells showed evidence of having epithelial-mesenchymal transition (EMT) shifting and endogenously co-expressed Snail. Furthermore, the knockdown of Snail expression significantly decreased the expression of ALDH1, inhibited cancer stem-like properties, and blocked the tumorigenic abilities of CD44⁺CD24^-ALDH1⁺ cells. Finally, in a xenotransplanted tumorigenicity study, we confirmed that the treatment effect of chemoradiotherapy for ALDH1⁺ could be improved by Snail siRNA. In summary, it is likely that ALDH1 is a specific marker for the cancer stem-like cells of HNSCC.

原文	英語
頁（從 - 到）	307-313
頁數	7
期刊	Biochemical and Biophysical Research Communications
卷	385
發行號	3
DOIs	https://doi.org/10.1016/j.bbrc.2009.05.048
出版狀態	已發佈 - 7月 31 2009
對外發佈	是

ASJC Scopus subject areas

生物物理學
生物化學
分子生物學
細胞生物學

UN SDG

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10.1016/j.bbrc.2009.05.048

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Chen, Y. C., Chen, Y. W., Hsu, H. S., Tseng, L. M., Huang, P. I., Lu, K. H., Chen, D. T., Tai, L. K., Yung, M. C., Chang, S. C., Ku, H. H., Chiou, S. H., & Lo, W. L. (2009). Aldehyde dehydrogenase 1 is a putative marker for cancer stem cells in head and neck squamous cancer. Biochemical and Biophysical Research Communications, 385(3), 307-313. https://doi.org/10.1016/j.bbrc.2009.05.048

@article{1f5d580e8236485eb292abf5e27966ed,

title = "Aldehyde dehydrogenase 1 is a putative marker for cancer stem cells in head and neck squamous cancer",

abstract = "Aldehyde dehydrogenase 1 (ALDH1) has been considered to be a marker for cancer stem cells. However, the role of ALDH1 in head and neck squamous cell carcinoma (HNSCC) has yet to be determined. In this study, we isolated ALDH1-positive cells from HNSCC patients and showed that these HNSCC-ALDH1+ cells displayed radioresistance and represented a reservoir for generating tumors. Based on microarray findings, the results of Western blotting and immunofluorescent assays further confirmed that ALDH1+-lineage cells showed evidence of having epithelial-mesenchymal transition (EMT) shifting and endogenously co-expressed Snail. Furthermore, the knockdown of Snail expression significantly decreased the expression of ALDH1, inhibited cancer stem-like properties, and blocked the tumorigenic abilities of CD44+CD24-ALDH1+ cells. Finally, in a xenotransplanted tumorigenicity study, we confirmed that the treatment effect of chemoradiotherapy for ALDH1+ could be improved by Snail siRNA. In summary, it is likely that ALDH1 is a specific marker for the cancer stem-like cells of HNSCC.",

keywords = "Aldehyde dehydrogenase 1, Cancer stem cell, Head and neck squamous cancer",

author = "Chen, {Yu Chih} and Chen, {Yi Wei} and Hsu, {Han Shui} and Tseng, {Ling Ming} and Huang, {Pin I.} and Lu, {Kai Hsi} and Chen, {Dow Tien} and Tai, {Lung Kuo} and Yung, {Ming Chi} and Chang, {Shih Ching} and Ku, {Hung Hai} and Chiou, {Shih Hwa} and Lo, {Wen Liang}",

note = "Funding Information: This study was supported by research grants from the National Science Council (96-3111-B-075-001-MY3), TVGH (V97B1-006,E1-008,F-001), the VGHUST Joint research Program, and the National Yang-Ming University (Ministry of Education, Aim for the Top University Plan), Taiwan. ",

year = "2009",

month = jul,

day = "31",

doi = "10.1016/j.bbrc.2009.05.048",

language = "English",

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pages = "307--313",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Aldehyde dehydrogenase 1 is a putative marker for cancer stem cells in head and neck squamous cancer

AU - Chen, Yu Chih

AU - Chen, Yi Wei

AU - Hsu, Han Shui

AU - Tseng, Ling Ming

AU - Huang, Pin I.

AU - Lu, Kai Hsi

AU - Chen, Dow Tien

AU - Tai, Lung Kuo

AU - Yung, Ming Chi

AU - Chang, Shih Ching

AU - Ku, Hung Hai

AU - Chiou, Shih Hwa

AU - Lo, Wen Liang

N1 - Funding Information: This study was supported by research grants from the National Science Council (96-3111-B-075-001-MY3), TVGH (V97B1-006,E1-008,F-001), the VGHUST Joint research Program, and the National Yang-Ming University (Ministry of Education, Aim for the Top University Plan), Taiwan.

PY - 2009/7/31

Y1 - 2009/7/31

N2 - Aldehyde dehydrogenase 1 (ALDH1) has been considered to be a marker for cancer stem cells. However, the role of ALDH1 in head and neck squamous cell carcinoma (HNSCC) has yet to be determined. In this study, we isolated ALDH1-positive cells from HNSCC patients and showed that these HNSCC-ALDH1+ cells displayed radioresistance and represented a reservoir for generating tumors. Based on microarray findings, the results of Western blotting and immunofluorescent assays further confirmed that ALDH1+-lineage cells showed evidence of having epithelial-mesenchymal transition (EMT) shifting and endogenously co-expressed Snail. Furthermore, the knockdown of Snail expression significantly decreased the expression of ALDH1, inhibited cancer stem-like properties, and blocked the tumorigenic abilities of CD44+CD24-ALDH1+ cells. Finally, in a xenotransplanted tumorigenicity study, we confirmed that the treatment effect of chemoradiotherapy for ALDH1+ could be improved by Snail siRNA. In summary, it is likely that ALDH1 is a specific marker for the cancer stem-like cells of HNSCC.

AB - Aldehyde dehydrogenase 1 (ALDH1) has been considered to be a marker for cancer stem cells. However, the role of ALDH1 in head and neck squamous cell carcinoma (HNSCC) has yet to be determined. In this study, we isolated ALDH1-positive cells from HNSCC patients and showed that these HNSCC-ALDH1+ cells displayed radioresistance and represented a reservoir for generating tumors. Based on microarray findings, the results of Western blotting and immunofluorescent assays further confirmed that ALDH1+-lineage cells showed evidence of having epithelial-mesenchymal transition (EMT) shifting and endogenously co-expressed Snail. Furthermore, the knockdown of Snail expression significantly decreased the expression of ALDH1, inhibited cancer stem-like properties, and blocked the tumorigenic abilities of CD44+CD24-ALDH1+ cells. Finally, in a xenotransplanted tumorigenicity study, we confirmed that the treatment effect of chemoradiotherapy for ALDH1+ could be improved by Snail siRNA. In summary, it is likely that ALDH1 is a specific marker for the cancer stem-like cells of HNSCC.

KW - Aldehyde dehydrogenase 1

KW - Cancer stem cell

KW - Head and neck squamous cancer

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Aldehyde dehydrogenase 1 is a putative marker for cancer stem cells in head and neck squamous cancer

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ASJC Scopus subject areas

UN SDG

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