@article{163ec5ea30ef4562b8cd63aee68b1c8b,
title = "Advanced glycosylated end products restrain the osteogenic differentiation of the periodontal ligament stem cell",
abstract = "Background/purpose: Many studies have confirmed that periodontal disease interacts with diabetes. The aim of this study was to examine whether the advanced glycosylated end products (AGEs), which are generated by diabetics, have important effects on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Materials and methods: In this study PDLSCs were isolated from the periodontal ligaments of extracted third molar teeth. The subjects were divided into two groups, which included the normal control group (N-PDLSCs) and the AGEs-stimulating group (A-PDLSCs). Changes of receptor of AGEs (RAGE) and cumulative ROS in PDLSCs were monitored by western blot and flow cytometry, respectively. Results: In the study AGEs noticeably inhibited the osteogenic differentiation of PDLSCs, with significant lower calcification nodules detected in A-PDLSCs (P < 0.01). RAGE expression level and ROS accumulation in A-PDLSCs were clearly higher than those in N-PDLSCs (P < 0.01). Conclusion: Our conclusions were that AGEs may cause the apoptosis of stem cells, which could lead to the disorder of bone differentiation function of PDLSCs.",
keywords = "AGEs (advanced glycosylated end products), Diabetes mellitus, PDLSCs (periodontal ligament stem cells), Periodontal disease, Prevention",
author = "Guo, {Zhu Ling} and Gan, {Shan Ling} and Cao, {Chun Yi} and Rao Fu and Cao, {Sheng Ping} and Chen Xie and Chen, {Jing Wei} and Alex Gibson and Xu Zheng and Teng, {Nai chia}",
note = "Funding Information: This research was funded by Hainan Provincial Natural Science Foundation of China , China ( 817330 ), Young Talents{\textquoteright} Science and Technology Innovation Project of Hainan Association for Science and Technology , China ( QCXM201817 ), Education Department of Hainan Province , China ( Hnky2017-31 ), Youth Development Fund Project of the First Affiliated Hospital of Hainan Medical University , China ( HYFYPY201702 ), Course Construction Project of Hainan Medical University , China ( HYJW201834 ), Medical Education Research Project of Medical Education Branch of Chinese Medical Association, China (2018B-N12003). Funding Information: This research was funded by Hainan Provincial Natural Science Foundation of China, China (817330), Young Talents? Science and Technology Innovation Project of Hainan Association for Science and Technology, China (QCXM201817), Education Department of Hainan Province, China (Hnky2017-31), Youth Development Fund Project of the First Affiliated Hospital of Hainan Medical University, China (HYFYPY201702), Course Construction Project of Hainan Medical University, China (HYJW201834), Medical Education Research Project of Medical Education Branch of Chinese Medical Association, China (2018B-N12003). Publisher Copyright: {\textcopyright} 2019 Association for Dental Sciences of the Republic of China",
year = "2019",
month = jan,
day = "1",
doi = "10.1016/j.jds.2019.03.007",
language = "English",
volume = "14",
pages = "146--151",
journal = "Journal of Dental Sciences",
issn = "1991-7902",
publisher = "中華牙醫學會",
number = "2",
}
