Adjusted mitochondrial DNA quantification in human embryos may not be applicable as a biomarker of implantation potential

Yi Xuan Lee, Chi Huang Chen, Shyr Yeu Lin, Yi Hui Lin, Chii Ruey Tzeng

研究成果: 雜誌貢獻文章同行評審

15 引文 斯高帕斯(Scopus)


Objective: To evaluate the feasibility of adjusted mitochondrial DNA quantification in human embryos as a biomarker for implantation potential. Design: Double-blind, observational, prospective analysis of an Asian population in a single university-affiliated in vitro fertilization center. A total of 1617 embryos derived from 380 infertile couples were collected. The DNA from blastomere biopsy (n = 99) or trophectoderm biopsy (n = 1518) were analyzed with next-generation sequencing. Results: The adjusted mtDNA quantification followed a non-normal distribution in both types of the embryos. When stratified by ploidy status, the adjusted mtDNA quantification was significantly higher in aneuploid trophectoderm than in euploid cells, but not in blastomeres. The adjusted mtDNA quantification of embryos showed significant but very weak positive correlation in total trophectoderm cells with maternal age (Spearman’s correlation, r = 0.095, p = 0.0028) but neither in blastomeres nor stratified by ploidy status. The median adjusted mtDNA quantification was also significantly higher in aneuploid blastocysts than in euploid ones while corrected with embryo morphology. Viable embryos did not contain significantly different quantities of adjusted mtDNA compared with nonviable embryos (implanted n = 103, non-implanted n = 164; median 0.00097 vs. 0.00088, p = 0.21) in 267 transferred blastocysts. Conclusion: Quantification of adjusted mitochondria DNA in human embryos was significantly lower in euploid blastocysts than in aneuploid blastocysts. However, no statistically significant differences regarding implantation outcome were evident. To our best knowledge, this study provides the largest scale and the first correlation data between mitochondria copy number and human embryo implantation potential in Asians.
頁(從 - 到)1855-1865
期刊Journal of Assisted Reproduction and Genetics
出版狀態已發佈 - 9月 1 2019

ASJC Scopus subject areas

  • 生殖醫學
  • 遺傳學
  • 婦產科
  • 發展生物學
  • 遺傳學(臨床)


深入研究「Adjusted mitochondrial DNA quantification in human embryos may not be applicable as a biomarker of implantation potential」主題。共同形成了獨特的指紋。