Adenosine triphosphate-evoked cytosolic calcium oscillations in human granulosa-luteal cells: Role of protein kinase C

Chen Jei Tai, Sung Keun Kang, Peter C.K. Leung

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18 引文 斯高帕斯(Scopus)


ATP has been shown to modulate progesterone production in human granulosa-luteal cells (hGLCs) in vitro. After binding to a G protein-coupled P2 purinergic receptor, ATP stimulates phospholipase C. The resultant production of diacylglycerol and inositol triphosphate activates protein kinase C (PKC) and intracellular calcium [Ca 2+] i mobilization, respectively. In the present study, we examined the potential cross-talk between the PKC and Ca 2+ pathway in ATP signal transduction. Specifically, the effect of PKC on regulating ATP-evoked [Ca 2+] i oscillations were examined in hGLCs. Using microspectrofluorimetry, [Ca 2+] i oscillations were detected in Fura-2 loaded hGLCs in primary culture. The amplitudes of the ATP-triggered [Ca 2+] i oscillations were reduced in a dose-dependent manner by pretreating the cells with various concentrations (1 nM to 10 μM) of the PKC activator, phorbol-12-myristate-13-acetate (PMA). A 10 μM concentration of PMA completely suppressed 10 μM ATP-induced oscillations. The inhibitory effect occurred even when PMA was given during the plateau phase of ATP evoked [Ca 2+] i oscillations, suggesting that extracellular calcium influx was inhibited. The role of PKC was further substantiated by the observation that, in the presence of a PKC inhibitor, bisindolylmaleimide I, ATP-induced [Ca 2+] i oscillations were not completely suppressed by PMA. Furthermore, homologous desensitization of ATP-induced calcium oscillations was partially reversed by bisindolylmaleimide I, suggesting that activated PKC may be involved in the mechanism of desensitization. These results demonstrate that PKC negatively regulates the ATP-evoked [Ca 2+] i mobilization from both intracellular stores and extracellular influx in hGLCs and further support a modulatory role of ATP and P2 purinoceptor in ovarian steroidogenesis.

頁(從 - 到)773-777
期刊Journal of Clinical Endocrinology and Metabolism
出版狀態已發佈 - 2001

ASJC Scopus subject areas

  • 內分泌學、糖尿病和代謝
  • 生物化學
  • 內分泌
  • 臨床生物化學
  • 生物化學(醫學)


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