Activation of topoisomerase II-mediated excision of chromosomal DNA loops during oxidative stress

Tsai Kun Li, Allen Y. Chen, Chiang Yu, Yong Mao, Huimin Wang, Leroy F. Liu

研究成果: 雜誌貢獻文章同行評審

150 引文 斯高帕斯(Scopus)

摘要

Hydrogen peroxide (H2O2), a reactive oxygen species (ROS), is known to induce oxidative stress and apoptosis. U937 cells treated with H2O2 were shown to produce high molecular weight (HMW) DNA fragments ~50 to 100 kb in size in <1 min. The formation of these HMW DNA fragments is reversible and shown to be mediated by DNA topoisomerase II (TOP2). Following this initial event, formation of irreversible HMW DNA fragments and nucleosomal ladders occurs. Our results thus demonstrate a potential role of TOP2 in oxidative damage of DNA and apoptotic cell death.

原文英語
頁(從 - 到)1553-1560
頁數8
期刊Genes and Development
13
發行號12
DOIs
出版狀態已發佈 - 6月 15 1999
對外發佈

ASJC Scopus subject areas

  • 遺傳學
  • 發展生物學

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