TY - JOUR
T1 - A tyrosine kinase profile of prostate carcinoma
AU - Robinson, Dan
AU - He, Feng
AU - Pretlow, Tom
AU - Kung, Hsing Jien
PY - 1996/6/11
Y1 - 1996/6/11
N2 - Tyrosine kinases play central roles in the growth and differentiation of normal and tumor cells. In this study, we have analyzed the general tyrosine kinase expression profile of a prostate carcinoma (PCA) xenograft, CWR2.2. We describe here an improved reverse transcriptase-PCR approach that permits identification of nearly 40 different kinases in a single screening; several of these kinases are newly cloned kinases and some are novel. According to this, there are 11 receptor kinases, 9 nonreceptor kinases, and at least 7 dual kinases expressed in the xenograft tissue. The receptor kinases include erbB2, erbB3, Ret, platelet-derived growth factor receptor, sky, nyk, eph, htk, sek (eph), ddr, and tkt. The nonreceptor kinases are lck, yes, abl, arg, jak1, tyk2, and etk/bmx. Most of the dual kinases are in the mitogen- activating protein (MAP) kinase-kinase (MKK) family, which includes MKK3, MKK4, MEK5, and a novel one. As a complementary approach, we also analyzed by specific reverse transcriptase-PCR primers the expression profile of erbB/epidermal growth factor receptor family receptors in a variety of PCA specimens, cell lines, and benign prostatic hyperplasia. We found that erbB1, -2, and -3 are often coexpressed in prostate tissues, but nut in erbB4. The information established here should provide a base line to study the possible growth and oncogenic signals of PCA.
AB - Tyrosine kinases play central roles in the growth and differentiation of normal and tumor cells. In this study, we have analyzed the general tyrosine kinase expression profile of a prostate carcinoma (PCA) xenograft, CWR2.2. We describe here an improved reverse transcriptase-PCR approach that permits identification of nearly 40 different kinases in a single screening; several of these kinases are newly cloned kinases and some are novel. According to this, there are 11 receptor kinases, 9 nonreceptor kinases, and at least 7 dual kinases expressed in the xenograft tissue. The receptor kinases include erbB2, erbB3, Ret, platelet-derived growth factor receptor, sky, nyk, eph, htk, sek (eph), ddr, and tkt. The nonreceptor kinases are lck, yes, abl, arg, jak1, tyk2, and etk/bmx. Most of the dual kinases are in the mitogen- activating protein (MAP) kinase-kinase (MKK) family, which includes MKK3, MKK4, MEK5, and a novel one. As a complementary approach, we also analyzed by specific reverse transcriptase-PCR primers the expression profile of erbB/epidermal growth factor receptor family receptors in a variety of PCA specimens, cell lines, and benign prostatic hyperplasia. We found that erbB1, -2, and -3 are often coexpressed in prostate tissues, but nut in erbB4. The information established here should provide a base line to study the possible growth and oncogenic signals of PCA.
KW - erbB kinase
KW - human xenograft
KW - oncogene
KW - reverse transcriptase-PCR
KW - signal transduction
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U2 - 10.1073/pnas.93.12.5958
DO - 10.1073/pnas.93.12.5958
M3 - Article
C2 - 8650201
AN - SCOPUS:0029954686
SN - 0027-8424
VL - 93
SP - 5958
EP - 5962
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 12
ER -