A tyrosine kinase profile of prostate carcinoma

Dan Robinson, Feng He, Tom Pretlow, Hsing Jien Kung

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198 引文 斯高帕斯(Scopus)


Tyrosine kinases play central roles in the growth and differentiation of normal and tumor cells. In this study, we have analyzed the general tyrosine kinase expression profile of a prostate carcinoma (PCA) xenograft, CWR2.2. We describe here an improved reverse transcriptase-PCR approach that permits identification of nearly 40 different kinases in a single screening; several of these kinases are newly cloned kinases and some are novel. According to this, there are 11 receptor kinases, 9 nonreceptor kinases, and at least 7 dual kinases expressed in the xenograft tissue. The receptor kinases include erbB2, erbB3, Ret, platelet-derived growth factor receptor, sky, nyk, eph, htk, sek (eph), ddr, and tkt. The nonreceptor kinases are lck, yes, abl, arg, jak1, tyk2, and etk/bmx. Most of the dual kinases are in the mitogen- activating protein (MAP) kinase-kinase (MKK) family, which includes MKK3, MKK4, MEK5, and a novel one. As a complementary approach, we also analyzed by specific reverse transcriptase-PCR primers the expression profile of erbB/epidermal growth factor receptor family receptors in a variety of PCA specimens, cell lines, and benign prostatic hyperplasia. We found that erbB1, -2, and -3 are often coexpressed in prostate tissues, but nut in erbB4. The information established here should provide a base line to study the possible growth and oncogenic signals of PCA.
頁(從 - 到)5958-5962
期刊Proceedings of the National Academy of Sciences of the United States of America
出版狀態已發佈 - 6月 11 1996

ASJC Scopus subject areas

  • 遺傳學
  • 多學科


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