A Transcriptomic Dataset of Liver Tissues from Global and Liver-Specific Bmal1 Knockout Mice

Guohao Han; Dan Li; Haisen Zhang; Chao Li; Luda Yang; Tiantian Ma; Xuerong Wang; Bairong Ma; Xiaodie Wu; Yang Tao; Ziang Wang; Aihua Wang; Hsu-Wen Chao; Yaping Jin; Huatao Chen

doi:10.1038/s41597-025-04545-4

A Transcriptomic Dataset of Liver Tissues from Global and Liver-Specific Bmal1 Knockout Mice

Guohao Han, Dan Li, Haisen Zhang, Chao Li, Luda Yang, Tiantian Ma, Xuerong Wang, Bairong Ma, Xiaodie Wu, Yang Tao, Ziang Wang, Aihua Wang, Hsu-Wen Chao, Yaping Jin, Huatao Chen

研究成果: 雜誌貢獻 › 文章 › 同行評審

摘要

The circadian clock regulates various physiological processes in mammals. The core circadian clock gene Bmal1 is crucial for maintaining the oscillations of the circadian clock system by controlling the rhythmic expression of numerous circadian clock-controlled genes. To explore the transcriptional changes associated with Bmal1 deletion in liver tissues, we collected liver tissues from global and liver-specific Bmal1 knockout mice, along with their respective control groups, at two circadian time points (CT2 and CT14) and used them for transcriptome sequencing analysis. Genotyping, locomotor activity analysis, and comprehensive quality control analyses, including base quality scores, GC content, and mapping rates, confirmed the high quality of sequencing data. Differential expression analysis and RT-qPCR validation confirmed the reliability and validity of the dataset. These data offer a valuable resource for researchers investigating the role of BMAL1 in liver physiology, pathology, and the broader field of circadian biology.

原文	英語
頁（從 - 到）	199
期刊	Scientific data
卷	12
發行號	1
DOIs	https://doi.org/10.1038/s41597-025-04545-4
出版狀態	已發佈 - 2月 3 2025

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10.1038/s41597-025-04545-4

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abstract = "The circadian clock regulates various physiological processes in mammals. The core circadian clock gene Bmal1 is crucial for maintaining the oscillations of the circadian clock system by controlling the rhythmic expression of numerous circadian clock-controlled genes. To explore the transcriptional changes associated with Bmal1 deletion in liver tissues, we collected liver tissues from global and liver-specific Bmal1 knockout mice, along with their respective control groups, at two circadian time points (CT2 and CT14) and used them for transcriptome sequencing analysis. Genotyping, locomotor activity analysis, and comprehensive quality control analyses, including base quality scores, GC content, and mapping rates, confirmed the high quality of sequencing data. Differential expression analysis and RT-qPCR validation confirmed the reliability and validity of the dataset. These data offer a valuable resource for researchers investigating the role of BMAL1 in liver physiology, pathology, and the broader field of circadian biology.",

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AU - Han, Guohao

AU - Li, Dan

AU - Zhang, Haisen

AU - Li, Chao

AU - Yang, Luda

AU - Ma, Tiantian

AU - Wang, Xuerong

AU - Ma, Bairong

AU - Wu, Xiaodie

AU - Tao, Yang

AU - Wang, Ziang

AU - Wang, Aihua

AU - Chao, Hsu-Wen

AU - Jin, Yaping

AU - Chen, Huatao

PY - 2025/2/3

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N2 - The circadian clock regulates various physiological processes in mammals. The core circadian clock gene Bmal1 is crucial for maintaining the oscillations of the circadian clock system by controlling the rhythmic expression of numerous circadian clock-controlled genes. To explore the transcriptional changes associated with Bmal1 deletion in liver tissues, we collected liver tissues from global and liver-specific Bmal1 knockout mice, along with their respective control groups, at two circadian time points (CT2 and CT14) and used them for transcriptome sequencing analysis. Genotyping, locomotor activity analysis, and comprehensive quality control analyses, including base quality scores, GC content, and mapping rates, confirmed the high quality of sequencing data. Differential expression analysis and RT-qPCR validation confirmed the reliability and validity of the dataset. These data offer a valuable resource for researchers investigating the role of BMAL1 in liver physiology, pathology, and the broader field of circadian biology.

AB - The circadian clock regulates various physiological processes in mammals. The core circadian clock gene Bmal1 is crucial for maintaining the oscillations of the circadian clock system by controlling the rhythmic expression of numerous circadian clock-controlled genes. To explore the transcriptional changes associated with Bmal1 deletion in liver tissues, we collected liver tissues from global and liver-specific Bmal1 knockout mice, along with their respective control groups, at two circadian time points (CT2 and CT14) and used them for transcriptome sequencing analysis. Genotyping, locomotor activity analysis, and comprehensive quality control analyses, including base quality scores, GC content, and mapping rates, confirmed the high quality of sequencing data. Differential expression analysis and RT-qPCR validation confirmed the reliability and validity of the dataset. These data offer a valuable resource for researchers investigating the role of BMAL1 in liver physiology, pathology, and the broader field of circadian biology.

KW - Animals

KW - ARNTL Transcription Factors/genetics

KW - Liver/metabolism

KW - Mice

KW - Mice, Knockout

KW - Transcriptome

KW - Circadian Clocks/genetics

KW - Circadian Rhythm/genetics

U2 - 10.1038/s41597-025-04545-4

DO - 10.1038/s41597-025-04545-4

M3 - Article

C2 - 39900971

SN - 2052-4463

VL - 12

SP - 199

JO - Scientific data

JF - Scientific data

IS - 1

ER -

A Transcriptomic Dataset of Liver Tissues from Global and Liver-Specific Bmal1 Knockout Mice

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