TY - JOUR
T1 - A screening platform for compounds with potential immuno-regulatory activities using human cord blood mononuclear cells
AU - Chen, Chia Jung
AU - Chia, Che Tsai
AU - Hsieh, Jung Feng
AU - Chien, Chichen Michael
AU - Wu, Tzu Hua
AU - Chen, Shui Tein
PY - 2006/12
Y1 - 2006/12
N2 - A systematic and combinatorial approach was adopted using human umbilical cord blood mononuclear cells (hUCB-MNCs) to screen for potential immuno-regulatory compounds. The hUCB-MNCs contain several types of immunogenic cells, which are a suitable material to mimic the in vivo immuno-response after drug treatment. hUCB-MNCs were treated with various natural products such as quercetin, astaxanthin, caffeic acid, bilobalide, eugenol, rutin and γ-dodecalactone (γ-DDL). Phenotypic expression analysis revealed that the subpopulation of CD3+ T cells, CD56+ NK cells and CD1a+ dendritic cells apparently increased after being treated with γ-DDL for 6 days. The expression of CD56 reached a maximum at 72 h with a dose-dependent relationship. The NK cells activation marker (CD69) also elevated following γ-DDL treatment. These results demonstrated that the γ-DDL has immuno-regulatory effects to enhance cord blood NK cells population and bioactivities. Such a high-throughput methodology using hUCB-MNCs may be an effective platform for systematically screening potential immuno-regulatory compounds.
AB - A systematic and combinatorial approach was adopted using human umbilical cord blood mononuclear cells (hUCB-MNCs) to screen for potential immuno-regulatory compounds. The hUCB-MNCs contain several types of immunogenic cells, which are a suitable material to mimic the in vivo immuno-response after drug treatment. hUCB-MNCs were treated with various natural products such as quercetin, astaxanthin, caffeic acid, bilobalide, eugenol, rutin and γ-dodecalactone (γ-DDL). Phenotypic expression analysis revealed that the subpopulation of CD3+ T cells, CD56+ NK cells and CD1a+ dendritic cells apparently increased after being treated with γ-DDL for 6 days. The expression of CD56 reached a maximum at 72 h with a dose-dependent relationship. The NK cells activation marker (CD69) also elevated following γ-DDL treatment. These results demonstrated that the γ-DDL has immuno-regulatory effects to enhance cord blood NK cells population and bioactivities. Such a high-throughput methodology using hUCB-MNCs may be an effective platform for systematically screening potential immuno-regulatory compounds.
KW - Flow cytometry
KW - High-throughput screening
KW - Human umbilical cord blood mononuclear cells
KW - Immuno-regulatory activities
KW - Natural products
KW - γ-dodecalactone
UR - http://www.scopus.com/inward/record.url?scp=33845240376&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845240376&partnerID=8YFLogxK
U2 - 10.2174/138620706779026033
DO - 10.2174/138620706779026033
M3 - Article
C2 - 17168683
AN - SCOPUS:33845240376
SN - 1386-2073
VL - 9
SP - 777
EP - 784
JO - Combinatorial Chemistry and High Throughput Screening
JF - Combinatorial Chemistry and High Throughput Screening
IS - 10
ER -