@article{75869fc2b65a4d5cb7bf450806a7432c,
title = "A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis",
abstract = "We designed a protein ladder (hereafter referred to as {"}Mega- tag{"}) that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R2= 0.997 ± 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis.",
keywords = "14-Epitope tag protein ladder, Anti-epitope tag antibodies, Epitope tags, Protein molecular estimation, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blot analysis",
author = "Kao, \{Chien Han\} and Cheng, \{Chiu Min\} and Chuang, \{Kuo Hsiang\} and Chuang, \{Chih Hung\} and Tzou, \{Shey Cherng\} and Cheng, \{Ta Chun\} and Hsieh, \{Yuan Chin\} and Liao, \{Kuang Wen\} and Wang, \{Yun Ming\} and Chang, \{Long Sen\} and Roffler, \{Steve R.\} and Chen, \{Fang Ming\} and Cheng, \{Tian Lu\}",
note = "Funding Information: This work was supported by grants from the National Research Program for Biopharmaceuticals, National Science Council, Taipei, Taiwan (NSC 100-2325-B-037-001 and 100-2321-B-037-006), the Department of Health, Executive Yuan, Taiwan (DOH100-TD-N-111-010 and DOH100-TD-C-111-002), and the Grant of Biosignature in Colorectal Cancers, Academia Sinica, Taiwan, and Kaohsiung Medical University Research Foundation (KMUER014 and KMUER012). ",
year = "2012",
month = dec,
day = "1",
doi = "10.1016/j.ab.2012.08.001",
language = "English",
volume = "431",
pages = "1--3",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1",
}