@article{86546ba954894d029884ab9bfcca433b,
title = "A positive regulatory feedback loop between eklf/klf1 and tal1/scl sustaining the erythropoiesis",
abstract = "The erythroid Kr{\"u}ppel-like factor EKLF/KLF1 is a hematopoietic transcription factor binding to the CACCC DNA motif and participating in the regulation of erythroid differentiation. With combined use of microarray-based gene expression profiling and the promoter-based ChIP-chip assay of E14.5 fetal liver cells from wild type (WT) and EKLF-knockout (Eklf−/−) mouse embryos, we identified the pathways and direct target genes activated or repressed by EKLF. This genome-wide study together with the molecular/cellular analysis of the mouse erythroleukemic cells (MEL) indicate that among the downstream direct target genes of EKLF is Tal1/Scl. Tal1/Scl encodes another DNA-binding hematopoietic transcription factor TAL1/SCL, known to be an Eklf activator and essential for definitive erythroid differentiation. Further identification of the authentic Tal gene promoter in combination with the in vivo genomic footprinting approach and DNA reporter assay demonstrate that EKLF activates the Tal gene through binding to a specific CACCC motif located in its promoter. These data establish the existence of a previously unknow positive regulatory feedback loop between two DNA-binding hematopoietic transcription factors, which sustains mammalian erythropoiesis.",
keywords = "Direct target genes, EKLF/ KLF1, Erythroid differentiation, Gene knockout, Genomic footprinting of Tal1 promoter, Global gene expression profiling, TAL1/ SCL, Transcriptional regulation",
author = "Hung, {Chun Hao} and Lee, {Tung Liang} and Huang, {Anna Yu Szu} and Yang, {Kang Chung} and Shyu, {Yu Chiau} and Wen, {Hau Ching} and Lu, {Mu Jie} and Shinsheng Yuan and Shen, {Che Kun James}",
note = "Funding Information: This research was supported by grants from the National Health Research Institute and the Academia Sinica (AS), Taipei, Taiwan, Republic of China. The works on bioinformatics were supported by Mathematics in Biology Group of Institute of Statistical Science, AS, and by grants from Academia Sinica (AS-100-TP-AB and AS-104-TP-A07). This research was also supported by grants from the Ministry of Science and Technology (MOST; grant 109-2311-B-182A-001). C.-K. James Shen was an NSC Frontier of Science Awardee and AS senior investigator awardee. Funding Information: Funding: This research was supported by grants from the National Health Research Institute and the Academia Sinica (AS), Taipei, Taiwan, Republic of China. The works on bioinformatics were supported by Mathematics in Biology Group of Institute of Statistical Science, AS, and by grants from Academia Sinica (AS-100-TP-AB and AS-104-TP-A07). This research was also supported by grants from the Ministry of Science and Technology (MOST; grant 109-2311-B-182A-001). C.-K. James Shen was an NSC Frontier of Science Awardee and AS senior investigator awardee. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = aug,
doi = "10.3390/ijms22158024",
language = "English",
volume = "22",
journal = "International journal of molecular sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "15",
}