TY - JOUR
T1 - A one- and two-dimensional NMR study of the B to Z transition of (m5dC-dG)3 in methanolic solution
AU - Feigon, Juli
AU - Wang, Andrew H.J.
AU - van der Marel, Gijs A.
AU - van Boom, Jacques H.
AU - Rich, Alexander
N1 - Funding Information:
Administration and the National Science Foundation. A.W. is supported by a grant from the American Cancer Society and by a grant and Contract N01-HB-1-3000 from the National Institutes of Health. J.F. is supported by a Damon Runyon-Walter Winchell Cancer Fund Fellowship, DRG-58S.
Funding Information:
ACKNOWLEDGMENTS J.F. would like to thank Drs. David Ruben and Ronald A. Haberkorn of the National Magnet Laboratory for their generous help and advice. We would also like to thank Drs. Gary Quigley and Michael McMillan for helpful discussion. The NMR ezperiments were performed at the NMR Facility for Biomolecular Research located at the Francis Bitter National Magnet Laboratory (Massachusetts Institute of Technology). The NMR Facility is supported by Grant RR00995 from the Division of Research Resources (National Institutes of Health) and by the National Science Foundation under Contract C-670. This research was also supported by grants to A.R. from the National Institutes of Health, the American Cancer Society, the National Aeronautics and Space
PY - 1984/1/25
Y1 - 1984/1/25
N2 - The deoxyribose hexanucleoside pentaphosphate (m5dC-dG)3 has been studied by 500 MHz 1H NMR in D2O (0.1 M NaCl) and in D2O/deuterated methanol mixtures. Two conformations, in slow equilibrium on the NMR time scale, were detected in methanolic solution. Two-dimensional nuclear Overhauser effect (NOE) experiments were used to assign the base and many of the sugar resonances as well as to determine structural features for both conformations. The results were consistent with the an equilibrium in solution between B-DNA and Z-DNA. The majority of the molecules have a B-DNA structure in low-salt D2O and a Z-DNA structure at high methanol concentrations. A cross-strand NOE between methyl groups on adjacent cytosines is observed for Z-DNA but not B-DNA. The B-DNA conformation predominates at low methanol concentrations and is stabilized by increasing temperature, while the Z-DNA conformation predominates at high methanol Concentrations and low temperatures. 31P NMR spectra gave results consistent with those obtained by 1H NMR. Comparison of the 31P spectra with those obtained on poly(dG-m5dC) allow assignment of the lower field resonances to GpC in the Z conformation.
AB - The deoxyribose hexanucleoside pentaphosphate (m5dC-dG)3 has been studied by 500 MHz 1H NMR in D2O (0.1 M NaCl) and in D2O/deuterated methanol mixtures. Two conformations, in slow equilibrium on the NMR time scale, were detected in methanolic solution. Two-dimensional nuclear Overhauser effect (NOE) experiments were used to assign the base and many of the sugar resonances as well as to determine structural features for both conformations. The results were consistent with the an equilibrium in solution between B-DNA and Z-DNA. The majority of the molecules have a B-DNA structure in low-salt D2O and a Z-DNA structure at high methanol concentrations. A cross-strand NOE between methyl groups on adjacent cytosines is observed for Z-DNA but not B-DNA. The B-DNA conformation predominates at low methanol concentrations and is stabilized by increasing temperature, while the Z-DNA conformation predominates at high methanol Concentrations and low temperatures. 31P NMR spectra gave results consistent with those obtained by 1H NMR. Comparison of the 31P spectra with those obtained on poly(dG-m5dC) allow assignment of the lower field resonances to GpC in the Z conformation.
UR - http://www.scopus.com/inward/record.url?scp=0021770223&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021770223&partnerID=8YFLogxK
U2 - 10.1093/nar/12.2.1243
DO - 10.1093/nar/12.2.1243
M3 - Article
C2 - 6694910
AN - SCOPUS:0021770223
SN - 0305-1048
VL - 12
SP - 1243
EP - 1263
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 2
ER -