TY - JOUR
T1 - A novel DNA structure induced by the anticancer bisplatinum compound crosslinked to a GpC site in DNA
AU - Yang, Danzhou
AU - van Boom, Stella S.G.E.
AU - Reedijk, Jan
AU - van Boom, Jacques H.
AU - Farrell, Nicholas
AU - Wang, Andrew H.J.
PY - 1995/7
Y1 - 1995/7
N2 - The bifunctional platinum compound, [{trans-PtCI(NH3)2}2(H2N(CH2)4NH2)]2+f forms a stable adduct with the self-complementary DNA oligomer CATGCATG, with the two platinum atoms coordinated at the N7 positions of the two symmetrical G4 nucleotides. The NMR-derived structure shows that the DNA octamer forms a novel hairpin structure with the platinated G4 residue adopting a syn conformation and the guanine base in the minor groove. Two such hairpins stack end-over-end and are linked together by the butanediamine tether to form a dumbbell structure. Such unusual structural distortion is different from that of the anticancer drug cisplatin-DNA adduct and may provide clues to explain the distinct biological activities of the two compounds.
AB - The bifunctional platinum compound, [{trans-PtCI(NH3)2}2(H2N(CH2)4NH2)]2+f forms a stable adduct with the self-complementary DNA oligomer CATGCATG, with the two platinum atoms coordinated at the N7 positions of the two symmetrical G4 nucleotides. The NMR-derived structure shows that the DNA octamer forms a novel hairpin structure with the platinated G4 residue adopting a syn conformation and the guanine base in the minor groove. Two such hairpins stack end-over-end and are linked together by the butanediamine tether to form a dumbbell structure. Such unusual structural distortion is different from that of the anticancer drug cisplatin-DNA adduct and may provide clues to explain the distinct biological activities of the two compounds.
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U2 - 10.1038/nsb0795-577
DO - 10.1038/nsb0795-577
M3 - Article
C2 - 7664126
AN - SCOPUS:0029094251
SN - 1072-8368
VL - 2
SP - 577
EP - 586
JO - Nature Structural Biology
JF - Nature Structural Biology
IS - 7
ER -