A novel detection platform for shrimp white spot syndrome virus using an ICP11-dependent immunomagnetic reduction (IMR) assay

Bing Hsien Liu; Yu Chen Lin; Chia Shin Ho; Che Chuan Yang; Yun Tsui Chang; Jui Feng Chang; Chun Yuan Li; Cheng Shun Cheng; Jiun Yan Huang; Yen Fu Lee; Ming Hung Hsu; Feng Chun Lin; Hao Ching Wang; Chu Fang Lo; Shieh Yueh Yang; Han Ching Wang

doi:10.1371/journal.pone.0138207

A novel detection platform for shrimp white spot syndrome virus using an ICP11-dependent immunomagnetic reduction (IMR) assay

Bing Hsien Liu, Yu Chen Lin, Chia Shin Ho, Che Chuan Yang, Yun Tsui Chang, Jui Feng Chang, Chun Yuan Li, Cheng Shun Cheng, Jiun Yan Huang, Yen Fu Lee, Ming Hung Hsu, Feng Chun Lin, Hao Ching Wang, Chu Fang Lo, Shieh Yueh Yang, Han Ching Wang

研究成果: 雜誌貢獻 › 文章 › 同行評審

9 引文斯高帕斯（Scopus）

摘要

Shrimp white spot disease (WSD), which is caused by white spot syndrome virus (WSSV), is one of the world's most serious shrimp diseases. Our objective in this study was to use an immunomagnetic reduction (IMR) assay to develop a highly sensitive, automatic WSSV detection platform targeted against ICP11 (the most highly expressed WSSV protein). After characterizing the magnetic reagents (Fe3O4 magnetic nanoparticles coated with anti ICP11), the detection limit for ICP11 protein using IMR was approximately 2 x 103 ng/ml, and the linear dynamic range of the assay was 0.1~1 x 106 ng/ml. In assays of ICP11 protein in pleopod protein lysates from healthy and WSSV-infected shrimp, IMR signals were successfully detected from shrimp with low WSSV genome copy numbers. We concluded that this IMR assay targeting ICP11 has potential for detecting the WSSV.

原文	英語
文章編號	e0138207
期刊	PLoS One
卷	10
發行號	9
DOIs	https://doi.org/10.1371/journal.pone.0138207
出版狀態	已發佈 - 9月 18 2015

ASJC Scopus subject areas

農業與生物科學 (全部)
生物化學、遺傳與分子生物學 (全部)
醫藥 (全部)

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10.1371/journal.pone.0138207

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Liu, B. H., Lin, Y. C., Ho, C. S., Yang, C. C., Chang, Y. T., Chang, J. F., Li, C. Y., Cheng, C. S., Huang, J. Y., Lee, Y. F., Hsu, M. H., Lin, F. C., Wang, H. C., Lo, C. F., Yang, S. Y., & Wang, H. C. (2015). A novel detection platform for shrimp white spot syndrome virus using an ICP11-dependent immunomagnetic reduction (IMR) assay. PLoS One, 10(9), [e0138207]. https://doi.org/10.1371/journal.pone.0138207

Liu, BH, Lin, YC, Ho, CS, Yang, CC, Chang, YT, Chang, JF, Li, CY, Cheng, CS, Huang, JY, Lee, YF, Hsu, MH, Lin, FC, Wang, HC, Lo, CF, Yang, SY & Wang, HC 2015, 'A novel detection platform for shrimp white spot syndrome virus using an ICP11-dependent immunomagnetic reduction (IMR) assay', PLoS One, 卷 10, 編號 9, e0138207. https://doi.org/10.1371/journal.pone.0138207

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abstract = "Shrimp white spot disease (WSD), which is caused by white spot syndrome virus (WSSV), is one of the world's most serious shrimp diseases. Our objective in this study was to use an immunomagnetic reduction (IMR) assay to develop a highly sensitive, automatic WSSV detection platform targeted against ICP11 (the most highly expressed WSSV protein). After characterizing the magnetic reagents (Fe3O4 magnetic nanoparticles coated with anti ICP11), the detection limit for ICP11 protein using IMR was approximately 2 x 103 ng/ml, and the linear dynamic range of the assay was 0.1~1 x 106 ng/ml. In assays of ICP11 protein in pleopod protein lysates from healthy and WSSV-infected shrimp, IMR signals were successfully detected from shrimp with low WSSV genome copy numbers. We concluded that this IMR assay targeting ICP11 has potential for detecting the WSSV.",

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AU - Li, Chun Yuan

AU - Cheng, Cheng Shun

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AU - Lee, Yen Fu

AU - Hsu, Ming Hung

AU - Lin, Feng Chun

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AU - Lo, Chu Fang

AU - Yang, Shieh Yueh

AU - Wang, Han Ching

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A novel detection platform for shrimp white spot syndrome virus using an ICP11-dependent immunomagnetic reduction (IMR) assay

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