TY - JOUR
T1 - A nanofluidic preconcentrator integrated with an aluminum-based nanoplasmonic sensor for Epstein-Barr virus detection
AU - Hsieh, Han Yun
AU - Luo, Jia Xian
AU - Shen, Yi Hong
AU - Lo, Shu Cheng
AU - Hsu, Yi Chiung
AU - Tahara, Hidetoshi
AU - Fan, Yu Jui
AU - Wei, Pei Kuen
AU - Sheen, Horn Jiunn
N1 - Funding Information:
This work was supported by the Ministry of Science and Technology (MOST) of Taiwan under grant numbers MOST 110-2636-E-038-004 , 110-2811-E-002-546 , 108-2221-E-002-174-MY3 , and 108-2221-E-001-019-MY3 , and by Academia Sinica under AS-BRPT-110-14 . We would also like to thank the NEMS Research Center, National Taiwan University, and the Scimage Club for facility support.
Funding Information:
This work was supported by the Ministry of Science and Technology (MOST) of Taiwan under grant numbers MOST 110-2636-E-038-004, 110-2811-E-002-546, 108-2221-E-002-174-MY3, and 108-2221-E-001-019-MY3, and by Academia Sinica under AS-BRPT-110-14. We would also like to thank the NEMS Research Center, National Taiwan University, and the Scimage Club for facility support.
Publisher Copyright:
© 2021
PY - 2022/3/15
Y1 - 2022/3/15
N2 - Epstein-Barr virus (EBV) positivity is one of the indexes for diagnosing nasopharyngeal carcinoma (NPC). Moreover, systemic inflammatory responses can easily be triggered in patients who are both EBV- and coronavirus disease 2019 (COVID-19)-positive. Development of rapid and highly sensitive EBV screening methods has become important. In this study, a nanofluidic preconcentrator integrated with a nanoslit Fano resonance biosensor was developed to detect latent membrane protein 1 (LMP1) for an EBV diagnosis. Through nanoimprinting and aluminum deposition, the low-cost nanoslit plasmonic sensing chip can be mass-produced. The nanoporous membrane was patterned on a sensing chip as an ion selective channel to concentrate LMP1 proteins. Anti-LMP1 immunoglobulin G was then modified to a sensing chip to immunosense LMP1. The Fano resonant spectrum of the capped nanoslit array produced a transmission peak followed by a dip. We recorded and analyzed the spectrum using four methods, including area, center of mass, peak value, and dip value methods. With preconcentration, a limit of detection (LOD) of 100 pg/ml and a sensing range of 100 pg/ml to 10 µg/ml was achieved using the peak value.
AB - Epstein-Barr virus (EBV) positivity is one of the indexes for diagnosing nasopharyngeal carcinoma (NPC). Moreover, systemic inflammatory responses can easily be triggered in patients who are both EBV- and coronavirus disease 2019 (COVID-19)-positive. Development of rapid and highly sensitive EBV screening methods has become important. In this study, a nanofluidic preconcentrator integrated with a nanoslit Fano resonance biosensor was developed to detect latent membrane protein 1 (LMP1) for an EBV diagnosis. Through nanoimprinting and aluminum deposition, the low-cost nanoslit plasmonic sensing chip can be mass-produced. The nanoporous membrane was patterned on a sensing chip as an ion selective channel to concentrate LMP1 proteins. Anti-LMP1 immunoglobulin G was then modified to a sensing chip to immunosense LMP1. The Fano resonant spectrum of the capped nanoslit array produced a transmission peak followed by a dip. We recorded and analyzed the spectrum using four methods, including area, center of mass, peak value, and dip value methods. With preconcentration, a limit of detection (LOD) of 100 pg/ml and a sensing range of 100 pg/ml to 10 µg/ml was achieved using the peak value.
KW - Epstein-Barr virus (EBV)
KW - Latent membrane protein 1
KW - Localized surface plasmon resonance
KW - Nanofluidic preconcentration
UR - http://www.scopus.com/inward/record.url?scp=85121990430&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85121990430&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2021.131327
DO - 10.1016/j.snb.2021.131327
M3 - Article
AN - SCOPUS:85121990430
SN - 0925-4005
VL - 355
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
M1 - 131327
ER -