Fibroblasts are the key components of tumor microenvironment (TME) and activity of these cells induces desmoplastic reactions in the spheroids. In this present work we co-cultured colon cancer cells (HCT-8) and fibroblast cells (NIH3T3) in a micro-well 3D (µ- well 3D) array cell culture chip and produced high throughput, even sized multicellular spheroids. The PDMS and contained 630 round wells, were coated with Pluroninc F-127 self-assembled molecules for rapid single organoids growth. The cross talk between colon cancer cells and fibroblast cells upregulated cancer associated fibroblast (CAF's) markers, which are co-related with increased stiffness and drug resistance in 3D co-cultured spheroids.
|名字||MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences|
|會議||25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021|
|城市||Palm Springs, Virtual|
|期間||10/10/21 → 10/14/21|