TY - GEN
T1 - A HIGH-THROUGHPUT-3D COLON CANCER TUMOR MICRO-SPHEROIDS FOR STUDYING TUMOUR MICROENVIRONMENT INDUCED STIFFNESS VARIATION AND DRUG RESISTANCE
AU - Goudar, Venkanagouda S.
AU - Lu, Long Sheng
AU - Koduri, Manohar Prasad
AU - Kumar, Ashish
AU - Tseng, Fan Gang
N1 - Funding Information:
We would like to thanks the financial support from MOST, Taiwan. REFERENCES [1] V G. Peddareddigari, D. Wang, R. N. DuBois, “The Tumor Microenvironment in Colorectal Carcinogenesis,” Cancer microenvironment, 3, 149-166, 2010. [2] Y. Fang, R. M. Eglen, “Three-Dimensional cell cultures in drug discovery,” SLAS Discov, 22, 456–472, 217. CONTACT * F.G. Tseng; phone: +886-937151305; fangangtseng@gmail.com
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Fibroblasts are the key components of tumor microenvironment (TME) and activity of these cells induces desmoplastic reactions in the spheroids. In this present work we co-cultured colon cancer cells (HCT-8) and fibroblast cells (NIH3T3) in a micro-well 3D (µ- well 3D) array cell culture chip and produced high throughput, even sized multicellular spheroids. The PDMS and contained 630 round wells, were coated with Pluroninc F-127 self-assembled molecules for rapid single organoids growth. The cross talk between colon cancer cells and fibroblast cells upregulated cancer associated fibroblast (CAF's) markers, which are co-related with increased stiffness and drug resistance in 3D co-cultured spheroids.
AB - Fibroblasts are the key components of tumor microenvironment (TME) and activity of these cells induces desmoplastic reactions in the spheroids. In this present work we co-cultured colon cancer cells (HCT-8) and fibroblast cells (NIH3T3) in a micro-well 3D (µ- well 3D) array cell culture chip and produced high throughput, even sized multicellular spheroids. The PDMS and contained 630 round wells, were coated with Pluroninc F-127 self-assembled molecules for rapid single organoids growth. The cross talk between colon cancer cells and fibroblast cells upregulated cancer associated fibroblast (CAF's) markers, which are co-related with increased stiffness and drug resistance in 3D co-cultured spheroids.
KW - 3D spheroids
KW - Co-culture
KW - Collagen
KW - ECM
KW - TME
KW - α-SMA
KW - µ- well
UR - http://www.scopus.com/inward/record.url?scp=85136982296&partnerID=8YFLogxK
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M3 - Conference contribution
AN - SCOPUS:85136982296
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 309
EP - 310
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -
