A crystallographic investigation of citrate synthase from pig and chicken heart muscle

Andrew H.J. Wang, M. L. Sherman, Alexander Rich

研究成果: 雜誌貢獻文章同行評審

5 引文 斯高帕斯(Scopus)

摘要

Citrate synthase (EC 4. 1. 3. 7.) from pig heart and chicken heart muscle can be crystallized from 10 mM phosphate buffer (pH 7. 0-7. 5) and 10-15% PEG4000 solution. The space group is P41212 with one subunit/asymmetric unit for the pig heart enzyme. The chicken heart citrate synthase purified from Blue-dextran as well as ATP-Sepharose affinity chromatography both crystallize in space group P21212 with one molecule/asymmetric unit, but they have different unit cell dimensions. The a-axis differs by about 17 Å between these two crystal forms, while b- and c-axis dimensions are virtually identical.

原文英語
頁(從 - 到)150-156
頁數7
期刊Biochemical and Biophysical Research Communications
82
發行號1
DOIs
出版狀態已發佈 - 5月 15 1978
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學

指紋

深入研究「A crystallographic investigation of citrate synthase from pig and chicken heart muscle」主題。共同形成了獨特的指紋。

引用此