摘要
Citrate synthase (EC 4. 1. 3. 7.) from pig heart and chicken heart muscle can be crystallized from 10 mM phosphate buffer (pH 7. 0-7. 5) and 10-15% PEG4000 solution. The space group is P41212 with one subunit/asymmetric unit for the pig heart enzyme. The chicken heart citrate synthase purified from Blue-dextran as well as ATP-Sepharose affinity chromatography both crystallize in space group P21212 with one molecule/asymmetric unit, but they have different unit cell dimensions. The a-axis differs by about 17 Å between these two crystal forms, while b- and c-axis dimensions are virtually identical.
原文 | 英語 |
---|---|
頁(從 - 到) | 150-156 |
頁數 | 7 |
期刊 | Biochemical and Biophysical Research Communications |
卷 | 82 |
發行號 | 1 |
DOIs | |
出版狀態 | 已發佈 - 5月 15 1978 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 生物物理學
- 生物化學
- 分子生物學
- 細胞生物學