981 Alterations of gene expression in papulopustular rosacea by whole transcriptome analysis

Y. Shih, J. Xu, A. Kumar, R. Li, A. Chang

研究成果: 雜誌貢獻文章同行評審


Rosacea is a common and incurable skin disorder. Identification of key molecular pathways found in rosacea may lead to new and better directions to treat this disease. The main objective was to identify differentially expressed genes in papulopustular rosacea skin in an unbiased fashion by whole transcriptome analysis. After IRB approval and written informed consent, rosacea skin biopsies from 6 individuals with untreated, papulopustular rosacea and their adjacent non-lesional skin were obtained. These samples were subjected to RNA extraction and RNA-sequencing (Illumina HiSeq 4000). Significantly altered genes were identified by pairwise comparison of rosacea versus adjacent non-lesional skin from the same individual by DESeq. Selected gene expression differences were confirmed by qRT-PCR. Gene Ontology (GO) term analysis was performed to identify biologic themes. Analysis identified 487 significantly altered genes (p
頁(從 - 到)S170
期刊Journal of Investigative Dermatology
發行號5, Supplement
出版狀態已發佈 - 2019