@article{20125c4078714d2b84b0454fb6b2c004,
title = "4D spatiotemporal modulation of biomolecules distribution in anisotropic corrugated microwrinkles via electrically manipulated microcapsules within hierarchical hydrogel for spinal cord regeneration",
abstract = "Although traditional 3D scaffolds or biomimetic hydrogels have been used for tissue engineering and regenerative medicine, soft tissue microenvironment usually has a highly anisotropic structure and a dynamically controllable deformation with various biomolecule distribution. In this study, we developed a hierarchical hybrid gelatin methacrylate-microcapsule hydrogel (HGMH) with Neurotrophin-3(NT-3)-loaded PLGA microcapsules to fabricate anisotropic structure with patterned NT-3 distribution (demonstrated as striped and triangular patterns) by dielectrophoresis (DEP). The HGMH provides a dynamic biomimetic sinuate-microwrinkles change with NT-3 spatial gradient and 2-stage time-dependent distribution, which was further simulated using a 3D finite element model. As demonstrated, in comparison with striped-patterned hydrogel, the triangular-patterned HGMH with highly anisotropic array of microcapsules exhibits remarkably spatial NT-3 gradient distributions that can not only guide neural stem cells (NSCs) migration but also facilitate spinal cord injury regeneration. This approach to construct hierarchical 4D hydrogel system via an electromicrofluidic platform demonstrates the potential for building various biomimetic soft scaffolds in vitro tailed to real soft tissues.",
keywords = "4D hydrogel, Biomimetic corrugation, Biomolecules distribution, Electromicrofluidic platform, Spinal cord injury repair",
author = "Chiang, {Min Yu} and Cheng, {Hung Wei} and Lo, {Yu Chun} and Wang, {Wei Chun} and Chang, {Shwu Jen} and Cheng, {Chu Hsun} and Lin, {Yu Chang} and Lu, {Huai En} and Sue, {Ming Wen} and Tsou, {Nien Ti} and Lo, {Yu Chun} and Li, {Ssu Ju} and Kuo, {Chao Hung} and Chen, {You Yin} and Huang, {Wei Chen} and Chen, {San Yuan}",
note = "Funding Information: This work is financially supported by Ministry of Science and Technology of Taiwan under Contract numbers of MOST 110-2321-B-010-006 , 109-2221-E-010-004-MY2 , 108-2221-E-009 -078 -MY3 , 108-2321-B-010 -008-MY2 , and 106-2221-E-009 -065 -MY3 . We also thank 7 T animal MRI Core Lab of the Neurobiology and Cognitive Science Center for technical and facility support and C. H. Hsieh and J. H. Chen of the Instrumentation Center for MRI experiments at National Taiwan University. Funding Information: This work is financially supported by Ministry of Science and Technology of Taiwan under Contract numbers of MOST 110-2321-B-010-006, 109-2221-E-010-004-MY2,108-2221-E-009 -078 -MY3, 108-2321-B-010 -008-MY2, and 106-2221-E-009 -065 -MY3. We also thank 7 T animal MRI Core Lab of the Neurobiology and Cognitive Science Center for technical and facility support and C. H. Hsieh and J. H. Chen of the Instrumentation Center for MRI experiments at National Taiwan University. Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",
year = "2021",
month = apr,
doi = "10.1016/j.biomaterials.2021.120762",
language = "English",
volume = "271",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier Science Ltd",
}