TY - JOUR
T1 - 3D scaffold with PCL combined biomedical ceramic materials for bone tissue regeneration
AU - Chern, Ming Jyh
AU - Yang, Liang Yo
AU - Shen, Yung Kang
AU - Hung, Jia Hsiang
N1 - Funding Information:
This work was supported by Taipei Medical University and National Taiwan University of Science and Technology. We also gratefully acknowledge the financial support from Taipei Medical University under contract TMU-NTUST-100-06. This research was also supported by Grant NSC 100-2314-B-038-011 (LYY) and Department of Health Grant DOH99-TD-B-111-003, Center of Excellence for Clinical Trial and Research in Neuroscience.
PY - 2013/12
Y1 - 2013/12
N2 - Three-dimensional porous biodegradable polymer scaffolds have been widely used for tissue engineering of bone repair or regeneration. The primary function of scaffolds is to provide structure support for the cells adhesion and proliferation. This study selects the Poly-e{open}-caprolactone (PCL) as material, NaCl mixed with hydroxyapatite (HA) or nano-aluminum oxide (nAl2O3) for porous scaffold. This study uses the solvent casting/particulate leaching method to fabricate the porous scaffold. The authors discuss the compression mechanical properties, physical properties (porosity, moisture content, contact angle) of a pure PCL, PCL/mHA, PCL/nHA and PCL/nAl2O3 scaffolds. In vitro cell culture is used for osteoblast cell (MG63) and the microculture tetrazolium test (MTT) is undertaken in the scaffold. The scaffolds are implanted to the femur of rats and histological examination is attempted after 2 weeks. The experimental results indicate that HA and nAl2O3 can improve the hydrophilic property. In conclusion, the PCL/nHA scaffold exhibits splendid in vivo biocompatibility and osteogenesis.
AB - Three-dimensional porous biodegradable polymer scaffolds have been widely used for tissue engineering of bone repair or regeneration. The primary function of scaffolds is to provide structure support for the cells adhesion and proliferation. This study selects the Poly-e{open}-caprolactone (PCL) as material, NaCl mixed with hydroxyapatite (HA) or nano-aluminum oxide (nAl2O3) for porous scaffold. This study uses the solvent casting/particulate leaching method to fabricate the porous scaffold. The authors discuss the compression mechanical properties, physical properties (porosity, moisture content, contact angle) of a pure PCL, PCL/mHA, PCL/nHA and PCL/nAl2O3 scaffolds. In vitro cell culture is used for osteoblast cell (MG63) and the microculture tetrazolium test (MTT) is undertaken in the scaffold. The scaffolds are implanted to the femur of rats and histological examination is attempted after 2 weeks. The experimental results indicate that HA and nAl2O3 can improve the hydrophilic property. In conclusion, the PCL/nHA scaffold exhibits splendid in vivo biocompatibility and osteogenesis.
KW - In vitro/In vivo
KW - Mechanical properties
KW - Porosity/Voids
KW - Scanning electron microscopy (SEM)
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U2 - 10.1007/s12541-013-0298-1
DO - 10.1007/s12541-013-0298-1
M3 - Article
AN - SCOPUS:84891115879
SN - 2234-7593
VL - 14
SP - 2201
EP - 2207
JO - International Journal of Precision Engineering and Manufacturing
JF - International Journal of Precision Engineering and Manufacturing
IS - 12
ER -