TY - GEN
T1 - 3D co-cultured multicellular spheroids on microfluidic chip for studying ecm-mediated drug resistance
AU - Goudar, Venkanagouda S.
AU - Lu, Long Sheng
AU - Koduri, Manohar Prasad
AU - Tseng, Fan Gang
N1 - Funding Information:
We would like to thanks the financial support from MOST, Taiwan.
Publisher Copyright:
© 2020 CBMS-0001
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - Among various ECM proteins, collagen type-I is a widely distributed extracellular matrix (ECM) protein expressed in different types of cancers and induces tumor growth and drug resistance. To prove this, in the present work we co-cultured colon cancer cells (HCT-8) and fibroblast cells (NIH3T3) in a PDMS based open welled micro 3D (μ-3D) cell culture chip. This co-culture model was employed to study the interaction of cancer cells and stromal cells and revealed that collagen treatment before chemotherapy can be a game-changer in a colon cancer treatment.
AB - Among various ECM proteins, collagen type-I is a widely distributed extracellular matrix (ECM) protein expressed in different types of cancers and induces tumor growth and drug resistance. To prove this, in the present work we co-cultured colon cancer cells (HCT-8) and fibroblast cells (NIH3T3) in a PDMS based open welled micro 3D (μ-3D) cell culture chip. This co-culture model was employed to study the interaction of cancer cells and stromal cells and revealed that collagen treatment before chemotherapy can be a game-changer in a colon cancer treatment.
KW - 3D spheroids
KW - Collagen type-I
KW - Colon cancer
KW - ECM
KW - μ-3D culture chip
UR - http://www.scopus.com/inward/record.url?scp=85098264482&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85098264482&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85098264482
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 853
EP - 854
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -
