@article{20ece1b2edc24af285d13938ce668914,
title = "κ-Carrageenan Gel Modified Mesoporous Gold Chronocoulometric Sensor for Ultrasensitive Detection of MicroRNA",
abstract = "Hydrogel-functionalized surface-based transducers demonstrate fluid-like kinetics, non-fouling properties, and superior biocompatibility. The integration of such properties of threedimensional (3D) micro- or macrostructure of hydrogels into a mesoporous platform provides a favorable moiety for incorporating biomolecule for adsorption or hybridization with a capture agent. Herein, we report a novel κ-carrageenan hydrogelcoated mesoporous gold (Au) electrode (abbreviated as MPGE/ gel) for chronocoulometric (CC) detection of microRNA (miRNA). The κ-carrageenan gel provides a 3D porous network on Au electrode surface to enable higher adsorption of target miRNA for CC interrogation of miRNA in presence of a redox molecule-ruthenium hexaammine (III) chloride ([Ru(NH3)6]3+, RuHex). Magnetically isolated and purified target miR-9-2 is adsorbed onto the MPGE via Au-RNA affinity interaction through the porous 3D network of the gel followed by the CC detection. The enhanced miRNA adsorption and electrocatalytic activity of MPGE/gel provide attomolar (50 aM) level of detection of miRNA with a dynamic range from 100pM to 10 aM. The good reproducibility (% RSD ≤ 5%, for n = 3) and high specificity of the developed biosensor demonstrates its excellent translational potential toward developing precisely controlled sensing devices for current clinical needs. ",
keywords = "Gold electrode, Mesoporous metals, MicroRNA sensor",
author = "Bidita Salahuddin and Masud, {Mostafa Kamal} and Shazed Aziz and Liu, {Chia Hung} and Nasim Amiralian and Aditya Ashok and Hossain, {S. M.Azad} and Hyeongyu Park and Wahab, {Md Abdul} and Amin, {Mohammed A.} and Chari, {M. Adharvana} and Rowan, {Alan E.} and Yusuke Yamauchi and Hossain, {Md Shahriar A.} and Kaneti, {Yusuf Valentino}",
note = "Funding Information: This study is supported by the JSPS fellowship to Mostafa Kamal Masud and the Advance Queensland Industry Research Fellowship (AQIRF043-2020RD3) to Yusuf Valentino Kaneti. The authors would like to thank Taif University Researchers Supporting Project number (TURSP-2020/03), Taif University, Taif, Saudia Arabia for supporting this project. Chia-Hung Liu would like to acknowledge funding support from Ministry of Science and Technology, Taiwan (MOST 110-2222-E-038-002). The MPGE fabrication is performed at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano-and micro-fabrication facilities for Australian researchers. Publisher Copyright: {\textcopyright} 2022 The Chemical Society of Japan.",
year = "2022",
doi = "10.1246/bcsj.20210286",
language = "English",
volume = "95",
pages = "198--207",
journal = "Bulletin of the Chemical Society of Japan",
issn = "0009-2673",
publisher = "Chemical Society of Japan",
number = "1",
}