Additional file 1 Statistical test results. Table S1: F and F-critical values from F-test to test the null hypothesis that two datasets’ variances are equal in terms of single amino acid, dipeptide and tripeptide composition. Table S2: p-values from the unpaired student T-test on single amino acid composition on positive group (18 TNF sequences) and negative group (non-TNF sequences) with the assumption that the variance are equal. Table S3: Top 20 dipeptides with the lowest p-values from the unpaired student T-test on dipeptide composition on positive group (18 TNF sequences) and negative group (non-TNF sequences) with the assumption that the variance are equal. Table S4: Top 20 tripeptides with the lowest p-values from the unpaired student T-test on tripeptide composition on positive group (18 TNF sequences) and negative group (non-TNF sequences) with the assumption that the variance are equal.