TY - JOUR
T1 - Z-DNA forms without an alternating purine-pyrimidine sequence in solution
AU - Feigon, Juli
AU - Wang, Andrew H.J.
AU - Van Der Marel, Gijs A.
AU - Van Boom, Jacques H.
AU - Rich, Alexander
PY - 1985
Y1 - 1985
N2 - Nuclear magnetic resonance spectra (proton and phosphorus-31) and ultraviolet absorption spectra of the DNA decamer d(br5CGbr 5CGATbr5CGbr5CG), in which the central two adenine-thymine base pairs are out of order with the rest of the purine-pyrimidine alternation sequence, indicate that under appropriate solvent conditions (high salt and methanol) the molecule undergoes a structural transition from a right-handed B-DNA conformation to a left-handed Z-DNA conformation. Measurements of the two-dimensional nuclear Overhauser effect on the decamer indicate that all of the guanines as well as the two equivalent thymines adopt the syn conformation.
AB - Nuclear magnetic resonance spectra (proton and phosphorus-31) and ultraviolet absorption spectra of the DNA decamer d(br5CGbr 5CGATbr5CGbr5CG), in which the central two adenine-thymine base pairs are out of order with the rest of the purine-pyrimidine alternation sequence, indicate that under appropriate solvent conditions (high salt and methanol) the molecule undergoes a structural transition from a right-handed B-DNA conformation to a left-handed Z-DNA conformation. Measurements of the two-dimensional nuclear Overhauser effect on the decamer indicate that all of the guanines as well as the two equivalent thymines adopt the syn conformation.
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U2 - 10.1126/science.4035359
DO - 10.1126/science.4035359
M3 - Article
C2 - 4035359
AN - SCOPUS:0022380477
SN - 0036-8075
VL - 230
SP - 82
EP - 84
JO - Science
JF - Science
IS - 4721
ER -