TY - JOUR
T1 - Xanthine oxidase and lipid peroxidation inhibition of Taiwan folkloric medicine factors affecting rhus semialata var. Roxburghiana activities against xanthine oxidase and ferrous iron-induced lipid peroxidation on mice liver mitochondria
AU - Tsai, Po Wei
AU - Lin, Pei Chin
AU - Yang, Ling Ling
AU - Liao, Ai Ho
AU - Wang, Bo Cheng
AU - Wu, Ming Shun
N1 - Funding Information:
The Alumni Foundation of Taipei Medical University, Taipei, Taiwan for the research grants; Department of Chinese Medicine and Pharmacy, Ministry of Health and Welfare (CCMP101-RD-007) for the partial research grant. A collaborating grant of Taipei Medical University and National Taipei University of Technology (USTP-NTUT-TMU-109-06) for the research grant. A collaborating grant of Taipei Medical University and National Taiwan University of Science and Technology (TMU-NTUST-103-10) for the research grant.
Publisher Copyright:
© 2020, Association of Pharmaceutical Teachers of India. All rights reserved.
PY - 2020/10
Y1 - 2020/10
N2 - Background: Gout, a disease characterized by recurrent inflammatory for urate deposit from overactive xanthine oxidase, is common among Taiwanese. Rhus semialata var. roxburghiana (RSR) grows wildly in Central Mountain of Taiwan and used by aboriginal Taiwanese as traditional medicine for gout and hepatitis. Methods: In this study, the factors such as variations in RSR extracts in terms of geographical sources, aerial parts and extraction solvents were investigated for antioxidant capabilities, phytochemical analysis and inhibitory effects against xanthine oxidase and lipid peroxidation using ferrous ion as ROS inducer on mice liver mitochondria. Results: The results showed that ML-n-hexane and NS-acetone gave the maximum XO inhibitions (IC50=16.74±0.74 μg/mL) and LPO inhibitions (IC50=8.40±0.35 μg/mL) in the liver mitochondria of mice, respectively. And ML-n-hexane and NS-acetone contained phenolic and flavonoids compounds for its potential target components against XO and LPO in this study. Conclusion: Overall, RSR could lead to a medicinal potential as a suitable candidate for the development of a natural anti-gout and liver damage protective agent that have yet to be conducted.
AB - Background: Gout, a disease characterized by recurrent inflammatory for urate deposit from overactive xanthine oxidase, is common among Taiwanese. Rhus semialata var. roxburghiana (RSR) grows wildly in Central Mountain of Taiwan and used by aboriginal Taiwanese as traditional medicine for gout and hepatitis. Methods: In this study, the factors such as variations in RSR extracts in terms of geographical sources, aerial parts and extraction solvents were investigated for antioxidant capabilities, phytochemical analysis and inhibitory effects against xanthine oxidase and lipid peroxidation using ferrous ion as ROS inducer on mice liver mitochondria. Results: The results showed that ML-n-hexane and NS-acetone gave the maximum XO inhibitions (IC50=16.74±0.74 μg/mL) and LPO inhibitions (IC50=8.40±0.35 μg/mL) in the liver mitochondria of mice, respectively. And ML-n-hexane and NS-acetone contained phenolic and flavonoids compounds for its potential target components against XO and LPO in this study. Conclusion: Overall, RSR could lead to a medicinal potential as a suitable candidate for the development of a natural anti-gout and liver damage protective agent that have yet to be conducted.
KW - Free radical scavenging
KW - Geographical source
KW - Liver mitochondria LPO inhibitor
KW - Rhus semialata var. Roxburghiana extracts
KW - Xanthine oxidase inhibitor
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U2 - 10.5530/ijper.54.4.201
DO - 10.5530/ijper.54.4.201
M3 - Article
AN - SCOPUS:85099060152
SN - 0019-5464
VL - 54
SP - 1062
EP - 1071
JO - Indian Journal of Pharmaceutical Education and Research
JF - Indian Journal of Pharmaceutical Education and Research
IS - 4
ER -
