Using Gelatin Scaffold With Coated Basic Fibroblast Growth Factor as a Transfer System for Transplantation of Human Neural Stem Cells

Y. W. Chen; S. H. Chiou; T. T. Wong; H. H. Ku; H. T. Lin; C. F. Chung; Sang-Hue Yen; C. L. Kao

doi:10.1016/j.transproceed.2006.02.084

Using Gelatin Scaffold With Coated Basic Fibroblast Growth Factor as a Transfer System for Transplantation of Human Neural Stem Cells

Y. W. Chen, S. H. Chiou, T. T. Wong, H. H. Ku, H. T. Lin, C. F. Chung, Sang-Hue Yen, C. L. Kao

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Gelatin scaffolds for ex vivo cell cultures are a promising development. These scaffolds can be used as three-dimensional skeletons for cell attachment and culture before transplantation. In this study, we isolated and cultivated neural stem cells from human brain tissues in serum-free medium (DMEM+F12 nutrient). Better neuron growth was observed using the tetrazolium assay (MTT) in the group when basic fibroblast growth factor (bFGF) was coated on the gelatin polymer scaffold. Further development of this nontoxic system may help the future development of transplantation of human neural stem cells.

Original language	English
Pages (from-to)	1616-1617
Number of pages	2
Journal	Transplantation Proceedings
Volume	38
Issue number	5
DOIs	https://doi.org/10.1016/j.transproceed.2006.02.084
Publication status	Published - Jun 2006
Externally published	Yes

ASJC Scopus subject areas

Surgery
Transplantation

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title = "Using Gelatin Scaffold With Coated Basic Fibroblast Growth Factor as a Transfer System for Transplantation of Human Neural Stem Cells",

abstract = "Gelatin scaffolds for ex vivo cell cultures are a promising development. These scaffolds can be used as three-dimensional skeletons for cell attachment and culture before transplantation. In this study, we isolated and cultivated neural stem cells from human brain tissues in serum-free medium (DMEM+F12 nutrient). Better neuron growth was observed using the tetrazolium assay (MTT) in the group when basic fibroblast growth factor (bFGF) was coated on the gelatin polymer scaffold. Further development of this nontoxic system may help the future development of transplantation of human neural stem cells.",

author = "Chen, {Y. W.} and Chiou, {S. H.} and Wong, {T. T.} and Ku, {H. H.} and Lin, {H. T.} and Chung, {C. F.} and Sang-Hue Yen and Kao, {C. L.}",

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AU - Chen, Y. W.

AU - Chiou, S. H.

AU - Wong, T. T.

AU - Ku, H. H.

AU - Lin, H. T.

AU - Chung, C. F.

AU - Yen, Sang-Hue

AU - Kao, C. L.

PY - 2006/6

Y1 - 2006/6

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AB - Gelatin scaffolds for ex vivo cell cultures are a promising development. These scaffolds can be used as three-dimensional skeletons for cell attachment and culture before transplantation. In this study, we isolated and cultivated neural stem cells from human brain tissues in serum-free medium (DMEM+F12 nutrient). Better neuron growth was observed using the tetrazolium assay (MTT) in the group when basic fibroblast growth factor (bFGF) was coated on the gelatin polymer scaffold. Further development of this nontoxic system may help the future development of transplantation of human neural stem cells.

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Using Gelatin Scaffold With Coated Basic Fibroblast Growth Factor as a Transfer System for Transplantation of Human Neural Stem Cells

Abstract

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